抗氧化胡萝卜籽肽的分离鉴定及活性表征

Isolation,identification and characterization of antioxidative carrot seed peptides

为从胡萝卜籽中纯化具有较高活性的抗氧化肽。采用凝胶过滤色谱和反向高效液相色谱法对胡萝卜籽蛋白酶解液进行分离纯化,以DPPH自由基清除率为指标,最终获得2个活性较强的抗氧化肽组分F3-a和F3-b。通过液质联用LC-ESI-MS/M分析得到其氨基酸序列分别为Lys-Asp-Asn-Phe-Leu-Phe(KDNFLF)和LeuPhe(LF)。此外,对2个多肽的ABTS自由基清除率、超氧阴离子自由基清除率和脂质过氧化抑制能力进行了测定,KDNFLF和LF均表现出较强的自由基清除活性。说明纯化的胡萝卜籽抗氧化肽具有良好的活性,可以作为潜在的抗氧化剂应用于食品工业及医药行业中。

To purify peptides with strong antioxidant activities,carrot seed protein hydrolysate was fractionated using size exclusion chromatography and it was further purified by RP-HPLC.Antioxidant activities of peptides were evaluated by DPPH radical scavenging activity.Two fractions,F3-a and F3-b,with higher DPPH scavenging activity were purified.Their sequences were identified as Lys-Asp-Asn-Phe-Leu-Phe(KDNFLF)and Leu-Phe(LF),respectively,by LC-ESI-MS/MS analysis.Moreover,the scavenging activities for ABTS radical and superoxide anion radical,as well as linoleic acid peroxidation inhibition activity were studied.Both KDNFLF and LF showed strong radical scavenging activities.These results suggested that the purified carrot seed antioxidant peptides possess good antioxidant activities and they could be used as potential antioxidants in the food and pharmaceutical industries.