百脉根起始结瘤相关转录因子间相互作用的鉴定

Identification of interaction between transcription factors involved in initiation of nodulation in Lotus japonicus

以模式豆科植物百脉根为材料,利用酵母双杂交和双分子荧光互补(bimolecular fluorescence complementation,BiFC)技术,研究调控起始结瘤基因表达的转录因子IPN2、NSP2、CYCLOPS、DELLA间的相互作用。结果显示,百脉根IPN2与DELLA、NSP2与DELLA、CYCLOPS与DELLA均有相互作用。表明IPN2、DELLA可能参与并形成一个大的蛋白复合物,网络调控豆科植物起始结瘤基因的时空表达。

Yeast two-hybrid assay and bimolecular fluorescence complementation assay(BiFC)were performed to identify the interaction of these transcription factors IPN2,NSP2,CYCLOPS,and DELLA involved in regulating the expression of the symbiotic gene during nodulation in the model legume Lotus japonicus.The results showed that Lotus DELLA interacted with IPN2,NSP2,and CYCLOPS,indicating that IPN2 and DELLA may form a large protein complex to regulate the spatial-temporal expression of the nodule inception gene in legumes.