邻菲啰啉并1,2,4-三嗪钴(Ⅲ)配合物的合成及其对小牛胸腺DNA的荧光识别

Synthesis of 1,2,4-Triazine-Phenanthroline Co (Ⅲ) Complexes and Their Fluorescence Recognition on Calf Thymus DNA

在4个邻菲啰啉并1,2,4-三嗪新型配体分子(ARTP1～ARTP4)合成的基础上,通过ARTP1～ARTP4与Co^(3+)配位,制备了4个钴(Ⅲ)配合物(Co-ARTP1～Co-ARTP4)。通过傅里叶变换红外光谱仪(FT-IR)、核磁共振波谱仪(NMR)和高分辨质谱(HRMS)仪和紫外-可见分光计(UV-Vis)等技术手段表征了ARTP1～ARTP4和Co-ARTP1～Co-ARTP4的结构和性能。结果表明,配合物的紫外吸收峰中出现减色效应,最大吸收峰红移。配合物以插入的方式与小牛胸腺DNA结合,结合常数Kb分别为4. 78×10~5、6. 52×10~5、5. 97×10~5和6. 01×10~5L/mol,表明配合物可作为潜在的CT-DNA荧光探针,为探索DNA与有机小分子的作用提供了重要的参考。

Four 1,2,4-triazine-phenanthroling derivatives(ARTP1~ARTP4) were designed and synthesized successfully.ARTP1~ARTP4 were selected to coordinate with Co^3+ to afford four novel complexes(Co-ARTP1~Co-ARTP4). The structures and properties of ARTP1~ARTP4 and Co-ARTP1~Co-ARTP4 were studied by means of Fourier transform infrared spectrometer(FT-IR), nuclear magnetic resonance(NMR), high resolution mass spectrometry(HRMS) and ultraviolet-visible spectroscopy(UV-Vis). As a result , the excitation peaks of the complexes are weakened and show red shift. The complexes interact with calf thymus DNA(CT-DNA) through the insert mode and the binding constants are Kb = 4.78×10^5 , 6.52×10^5 , 5.97×10^5 and 6.01×10^5 L/mol, respectively, which indicates that the complexes are expected to be the DNA fluorescence probes. It provides the important theory references for further study on the interaction between small organic molecules with DNA.