5%唑啉·炔草酯乳油高效液相色谱分析

Analysis of Pinoxaden+Clodinafop-propargyl 5% EC by HPLC

采用高效液相色谱法,以甲醇+水为流动相进行梯度洗脱,柱温为35℃,经C18色谱柱分离,在230 nm波长下对5%唑啉·炔草酯乳油中的有效成分进行分离和定量分析。结果表明,唑啉草酯和炔草酯的线性相关系数分别为0.999 8和0.999 1,变异系数分别为1.20%和1.42%,平均回收率分别为99.70%和98.51%。

A method was established for determination of pinoxaden+clodinafop-propargyl 5%EC by HPLC on C18 column with UV at 230 nm.Gradient elution was applied with mobile phase methanol+water solvent at 35℃.The results showed that the linear correlation coefficients of pinoxaden and clodinafop-propargyl were 0.999 8 and 0.999 1,the variation coefficients were 1.20%and 1.42%,the average recoveries were 99.70%and 98.51%,respectively.