华支睾吸虫生活史室内微生态建立

Establishment of indoor micro-ecological environment for life cycle of Clonorchis sinensis

目的构建华支睾吸虫生活史的室内微小生态环境。方法麦穗鱼饲养池120cm×60cm×50cm,隔成3个区间,纹沼螺饲养盆66cm×40cm×18cm,底部铺塘泥,种植水韭草,24h充氧和抽水循环,12h光照,每2周换水1/2;将华支睾吸虫虫卵和纹沼螺一起放入平皿内感染8h,螺与虫卵比例约1∶50,每周重复1次,连续3次;将收集的尾蚴与麦穗鱼一起放入大烧杯进行尾蚴感染,加水1 000mL,充氧过夜。结果纹沼螺和麦穗鱼均能在饲养池内正常生长与繁殖;纹沼螺感染率约4.9%,麦穗鱼形成的囊蚴数与用于感染的尾蚴数之比约30.65%。结论室内微型华支睾吸虫生活史生态环境构建成功。

The aim of our study is to establish indoor micro-ecological environment for the life cycle of Clonorchis sinensis.The aquarium for breeding Pseudorasbora parva was 120 cm×60 cm×50 cm in size,and be divided into three sections;the basin for breeding Parafossarulus striatulus was 66 cm×40 cm×18 cm,and the bottom was covered with mud,and the Valisneria spiralis was planted,24 hours oxygenation and pumping circulation,12 hours of light,and change the water in half every 2 weeks.Place the eggs and snails together in a plate to keep 8 hours for infection,the ratio of the snail to eggs was about 1∶50,repeat once a week for 3 times in a row.The collected cercariae and Pseudorasbora parva were put into a large beaker with 1 000 mL water for infection overnight.Results showed that both the Parafossarulus striatulus and Pseudorasbora parva could grow and reproduce normally in the feeding environment.The infection rate of Parafossarulus striatulus was 4.9%,and the rate of the total number of metacercariae formed compared to the number of cercariae used for infection was about 30.65%.In conclusion,the indoor microenvironment of the life cycle of Clonorchis sinensis is successfully to establish.