MMP-1、TIMP-1在不同年龄患者及不同退变程度椎间盘纤维环和髓核组织中的表达

Expression of MMP-1 and TIMP-1 annulus fibrosus and nucleus pulposus of degenerated intervertebral discs in different degrees and age

目的探究基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)、组织金属蛋白酶抑制因子-1(tissue inhibitor of metalloproteinase-1,TIMP-1)在不同年龄患者及不同退变程度椎间盘纤维环和髓核组织中的表达。方法选择2016年1月至2017年1月于本院行腰椎间盘切除术的腰椎间盘突出症患者120例为研究对象,将其中40例中年患者纳入中年组,40例老年患者纳入老年组,40例青年患者纳入青年组,采用免疫组织化学法检测并比较三组患者椎间盘纤维环和髓核组织中MMP-1、TIMP-1阳性表达率,同时比较各组内纤维环和髓核组织中MMP-1、TIMP-1阳性表达率。结果纤维环和髓核组织中MMP-1和TIMP-1阳性表达率均为老年组>中年组>青年组(P_均<0.05),且三组患者髓核组织中MMP-1、TIMP-1阳性表达率均显著高于其在纤维环组织中的阳性表达率(P_均<0.05)。三组患者髓核及纤维环组织中MMP-1、TIMP-1阳性表达率均为游离型>脱出型>突出型(P_均<0.05)。Spearman相关性分析显示:MMP-1和TIMP-1阳性表达率与患者年龄、椎间盘退变程度均呈正相关(P_均<0.05)。结论 MMP-1和TIMP-1在退变椎间盘组织中的表达与患者年龄及椎间盘退变程度均呈正相关,且在髓核处较纤维环处变化更明显,MMP-1和TIMP-1可作为判断椎间盘退变程度的重要标志物。

Objective To investigate the expression of matrix metalloproteinase 1(MMP-1)and tissue inhibitor of metallop roteinase 1(TIMP-1)in annulus fibrosus and nucleus pulposus of degenerated intervertebral discs in different degrees and age.Method From January 2016 to January 2017,120 patients with lumbar disc herniation treated by lumbar discectomy were divided into middleaged group(n=40),old-age group(n=40)and youth group(n=40).Immunohistochemistry was used to detect and compare the expression of MMP-1 and TIMP-1 of three groups,and to compare the expression of MMP-1 and TIMP-1 in different degeneration of intervertebral disc.Result The positive rates of MMP-1 and TIMP-1 expression in annulus fibrosus and nucleus pulposus were:elderly group>middle-aged group>young group(Pall<0.05).The positive rates of MMP-1 and TIMP-1 in nucleus pulposus of the three groups were significantly higher than those in annulus fibrosus(Pall<0.05).The positive rates of MMP-1 and TIMP-1 in nucleus pulposus and annulus fibrosus of the three groups were:free type>exfoliative type>prominent type(Pall<0.05).Spearman correlation analysis showed that the positive rates of MMP-1 and TIMP-1 were positively correlated with age and degree of degeneration(Pall<0.05).Conclusion The positive rate of MMP-1 and TIMP-1 expression are positively corre lated with ages and the degree of degeneration.The expression in nucleus pulposus is higher than that in annulus fibrosus.It illustrates that MMP-1 and TIMP-1 are involved in the degeneration of intervertebral disc,and can be the important indicator of prognosis.