虫生真菌Isaria cateniannulata次级代谢产物及抗肿瘤活性研究

Study on Secondary Metabolites and Antitumor Activities of Entomopathogenic Fungus Isaria cateniannulata

[目的]对虫生真菌环链棒束孢(Isaria cateniannulata)的次级代谢产物进行分离并研究其抗肿瘤活性。[方法]采用溶剂萃取、硅胶柱色谱、凝胶Sephadex LH-20等方法进行分离纯化,根据理化性质和波谱技术对分离得到的化合物进行结构鉴定,利用MTT法对化合物的抗肿瘤活性进行测试。[结果]从虫生真菌Isaria cateniannulata中共分离得到8个化合物,分别为β-麦角甾醇、豆甾-4-烯-3-酮、5α,8β-过氧-(22E,24R)-麦角甾-6,22-二烯3β-醇、4-methyl-5,6-dihydro-2H-pyran-2-one、(R)-甲羟戊酸内酯、对羟基苯甲醛、β-石竹烯和石竹烯氧化物。除化合物4外,其余化合物均是从该种中首次分离得到。抗肿瘤结果显示,化合物7、8对肿瘤细胞具有一定的抑制能力。[结论]该研究为新的抗肿瘤药物开发方面提供了广阔空间。

[Objective]The research aimed to isolate the secondary metabolites of entomogenous fungus Isaria cateniannulata and to study their anti-tumor activity.[Method]Compounds were isolated by the methods of solvent extraction,silica gel column chromatography,sephadex LH-20,and then the structures were identified by physicochemical properties and spectrum technology.The anti-tumor activity of the isolated compounds was tested using MTT method.[Result]Eight compounds were isolated from the entomopathogenic fungus Isaria cateniannulata,which wereβ-ergosterol,myristate-4-en-3-one,5α,8β-peroxy-(22E,24R)-ergoside-6,22-diene 3β-alcohol,4-methyl-5,6-dihydro-2H-pyran-2-one,(R)-mevalonate,p-hydroxybenzaldehyde,β-caryophyllene and caryophyllene oxid.All the compounds except compound 4 were firstly isolated from Isaria cateniannulata.The anti-tumor result indicated that compounds 7 and 8 presented a certain inhibitory ability to tumor cells.[Conclusion]The study provides a broad space for new anti-cancer drug development.