芦荟大黄素对培养人成纤维细胞Smad 2与Ⅰ型胶原表达及细胞周期的影响

Effect of Aloe-emodin on the Expression of Smad 2 and Collagen Ⅰ and Cell Cycle in the Fibroblasts in Vitro

目的:探讨中药大黄提取物芦荟大黄素对培养人成纤维细胞Smad 2、Ⅰ型胶原蛋白表达及细胞周期的影响,分析芦荟大黄素对成纤维细胞的调控作用及作用机制。方法:体外培养人成纤维细胞,用芦荟大黄素进行干扰,分为对照组(CG)、低剂量组(50-TG)、中剂量组(100-TG)、高剂量组(500-TG),观察细胞生长特征,并用量子点免疫荧光技术检测Smad 2、Ⅰ型胶原的表达,流式细胞仪检测的细胞周期。结果:(1)干扰48h后,实验组Smad 2表达的荧光强度,与对照组之间差异有统计学意义(P <0. 05);(2)实验组Ⅰ型胶原表达,与对照组之间差异有统计学意义(P <0. 05);(3)流式细胞仪检测细胞周期结果显示,对照组、实验组G1期细胞所占比例比较,差异有统计学意义(P <0. 05)。结论:芦荟大黄素对培养成纤维细胞的Smad 2、Ⅰ型胶原蛋白表达具有调控作用,并能影响其细胞周期和细胞的活性,可能是含有芦荟大黄素的中药如芦荟、大黄等调控成纤维细胞功能的主要机制之一。

Objective:To explore the effect of the aloe-emodin from traditional Chinese medicine on the expression of Smad 2,collagenⅠin fibroblasts and cell cycle of fibroblasts from human,and study role of adjustment and the mechanism of aloe-emodin on fibroblasts in vitro.Methods:Culture human fibroblasts in vitro and interfere the cells with aloe-emodin,then detect the expression of Smad 2,collagenⅠwith immunofluorescent,and cell cycle with flow cytometry(FCM).Results:(1)After 48 hours,there is significant difference of immunofluorescent of the expression of Smad 2 between the control group and the treatment group(P<0.05);(2)after 48 hours,there is significant difference of immunofluorescent of the expression of collagenⅠbetween the control group and the treatment group(P<0.05);(3)there is significant difference of the rate of G1 cells between the control group and the treatment group(P<0.05).Conclusion:Aloe-emodin have the role of adjustment the cell cycle and expression of collagenⅠ,collagenⅢin the fibroblasts in vitro,that may be the main mechanisms of the aloe-emodin from traditional Chinese medicine to regulating the function of fibroblasts.