靶向前列腺癌的纳米超声造影剂的研究

Study of Novel Nano Ultrasound Contrast Agent Targeted at Prostate Cancer

目的:对前期研究的适体修饰的多壁碳纳米管(MWCNTs)纳米靶向造影剂作进一步的研究探讨。方法:对纳米超声造影剂采用激光共聚焦显微镜及流式细胞仪进行体外细胞摄取评价。采用裸鼠移植瘤模型对造影剂进行体内靶向性评价、体内显影效果与代谢分布评价。结果:体外细胞学研究表明,该造影剂可被细胞摄取,且分布于细胞质中。体内靶向性评价结果表明,该造影剂具有肿瘤靶向性,且其在体内显影效果较好。结论:该新型造影剂能够靶向显影肿瘤部位,具有良好地临床应用的潜能。

Objective:To further study the nano-targeted contrast agent based on previous study.Methods:In vitro cell uptake evaluation of MWCNTs nano-ultrasound contrast agent was performed by laser confocal microscope and flow cytometry.The model of nude mouse transplantation was used to evaluate the targeting of contrast agent in vivo and to evaluate the development effect and metabolic distribution in vivo.Results:In vitro results showed that the contrast agent had good cell uptake.In vivo results showed that the contrast agent was tumor targeting and had good development effects.Conclusion:The new contrast agent can target tumor site and has potential of good clinical applications.