有机碳对氨氧化细菌及氨氧化古菌的影响

Effects of organic carbon on ammonia-oxidizing bacteria and ammonia-oxidizing archaea

氮是水生态系统初级生产力的限制性生源要素,也是造成黑臭水体的重要污染物。微生物是氮循环的驱动泵,硝化作用及反硝化是微生物的特有过程,也是氮素生物地球化学循环以及氮素去除过程中最为关键的步骤。通过分析体系中氮转化、氨氧化古菌(AOA)及氨氧化细菌(AOB)数量和活性对不同有机炭的响应发现,有机碳的添加增大了氨氧化古菌在氨氧化菌群中的比例,而氨氧化细菌比氨氧化古菌对铵根离子的降低更为敏感体系的潜在硝化速率与氨氧化细菌的氨单加氧酶基因拷贝数具有正向相关性。本研究结果对如何通过促进水体自身氮转化活性而快速削减水体中氨氮提供了指导。

Nitrogen is a limiting factor of primary productivity in aquatic ecosystem,and also an important pollutant causing black and odorous water.Microorganism is the driving pump of nitrogen cycle.Nitrification and denitrification are the unique processes of microorganism,and also the most critical steps in nitrogen biogeochemical cycle and nitrogen removal process.By analyzing the response of nitrogen transformation,the quantity and activity of ammoniaoxidizing archaea(AOA)and ammonia-oxidizing bacteria(AOB)to different organic carbon,it was found that the addition of organic carbon increased the proportion of ammonia-oxidizing archaea in ammonia-oxidizing bacteria,and that ammonia-oxidizing bacteria were more sensitive to the reduction of ammonium ion than ammonia-oxidizing archaea.The potential nitrification rate of the system is positively correlated with the ammonia monooxygenase gene copy number of the ammonia-oxidizing bacteria.The results provided guidance on how to rapidly reduce ammonia nitrogen in water by promoting the nitrogen conversion activity of water body.