牦牛肉品质相关的去饱和脂肪酶1基因表达差异分析

Analysis of the Expression Differences of Stearoyl-CoA Desaturase 1 Gene with Related Quality of Yak Meat

检测牦牛组织中去饱和脂肪酶1(stearoyl-CoA desaturase 1,SCD1)基因表达量,分别选择1月份、4月份、9月份年龄相近,取其肝脏、肾脏、结肠、背最长肌4个组织,采用实时荧光定量聚合酶链式反应(real-time fluorescent quantitative polymerase chain reaction,RT-PCR)技术和酶联免疫反应测定牦牛不同组织中SCD1的表达量和含量。结果表明:以肝脏为对照组,SCD1基因在牦牛肝脏、肾脏、结肠、背最长肌4个组织中均有表达,且SCD1基因表达量和蛋白含量均在牦牛背最长肌中最高,差异显著(P<0.05)。不同月份间,1月份牦牛SCD1基因表达量和蛋白含量都低于9月份和4月份。说明SCD1基因在牦牛不同组织均有表达,且在寒冷季节表达量较低。

To detect the expression of stearoyl-CoA desaturase 1(SCD1)gene in yak tissue,the samples were selected in January,April,September and taked the liver,kidney,colon,longissimus dorsi.There were real-time fluorescent quantitative polymerase chain reaction(RT-PCR)technology and enzyme-linked immunosorbent assay measuring yak SCD1 expression and protein content in different tissues.The results showed that SCD1 gene was expressed in all four tissues of yak liver,kidney,colon and dorsal longest muscle,and SCD1 gene expression and content were highest in the longissimus dorsi muscle of yak with significant difference(P<0.05).The expression and protein content of SCD1 gene in yak were lower in January than that of sample in September and in April.The results indicated that SCD1 gene was expressed in different tissues of yak and had a low expression in cold season.