牛蒡子苷元对人前列腺癌PC3细胞侵袭、迁移的影响

Effect of Arctigenin on Invasion and Migration of Human Prostate Cancer PC3 Cells

目的研究牛蒡子苷元对PC3细胞侵袭、迁移的影响。方法体外培养PC3细胞,采用MTT法检测牛蒡子苷元(ARG)对PC3细胞增殖抑制率,Transwell法检测细胞侵袭,划痕实验检测细胞迁移,以RT-PCR法检测侵袭、迁移标志物基质金属蛋白酶9(MMP-9),MMP-2,CXC趋化因子受体4(CXCR4)的基因表达,并以Western blot法检测其蛋白表达。结果 ARG可显著抑制PC3细胞增殖,且具有时间依赖性;与空白对照组比较,ARG 10μmol/L组和2.5μmol/L组均能有效抑制PC3细胞侵袭,其侵袭抑制率分别为73.5%和51.3%;10μmol/L组和2.5μmol/L组划痕修复率明显低于空白对照组,其差异有统计学意义(P<0.01,P<0.05);ARG可降低MMP-9,MMP-2,CXCR4的基因与蛋白表达,且具有浓度依赖性。结论 ARG在体外可抑制PC3细胞的侵袭、迁移,下调细胞中MMP-9、MMP-2和CXCR4的基因与蛋白表达。

Objective To investigate the effect of arctigenin on the invasion and migration of human prostate cancer PC3 cells. Methods PC3 cells were cultured in vitro, the inhibition rate of arctigenin (ARG) on the proliferation of PC3 cells was detected by MTT assay, the cell invasion was detected by Transwell assay, and the cell migration was detected by scratch test. The expression of MMP-9, MMP-2 and CXCR4 genes, which were used as biomarkers for reflecting cell invasion and migration, were detected by RT-PCR and their protein expression were detected by Western blot. Results ARG could markedly inhibit the proliferation of PC3 cells, appeared in time-dependent pattern. 10 μmol/ L and 2.5 μmol/L of ARG effectively inhibited the invasion of PC3 cells, the rate of inhibition were 73.5 % and 51.3 %, respectively. The scratch repair rates in 10 and 2.5 μmol/L groups were significantly lower than those in the control group (P<0.01, P<0.05). ARG could reduce the gene expression and protein expression of MMP-9, MMP- 2 and CXCR4 by the concentration-dependent manner. Conclusion ARG can significantly inhibit the invasion and migration of PC3 cells and reduce the gene expression and protein expression of MMP-9, MMP-2 and CXCR4.