狼疮性肾炎患者血清和尿液中IL-32和KIM-1的表达水平及临床意义

Expression and clinical significance of IL-32 and KIM-1 in serum and urine of patients with lupus nephritis

目的探讨狼疮性肾炎患者血清和尿液中白细胞介素-32(IL-32)和肾损伤分子1抗体(KIM-1)的表达水平及临床意义。方法选择狼疮性肾炎(LN)患者166例,分为非活动期LN组86例,活动期LN组80例,选择同期门诊正常体检者70例为对照组;测定3组血清和尿液中IL-32和KIM-1水平。结果非活动期LN组、活动期LN组血清和尿液IL-32、KIM-1水平高于对照组,活动期LN组血清和尿液IL-32、KIM-1水平高于非活动期LN组(t=12. 45、15. 37、15. 87、14. 96、13. 06、15. 43、14. 95、17. 15,P <0. 05);非活动期LN组、活动期LN组血清BUN、Crea水平高于对照组,C3、C4水平低于对照组;活动期LN组血清BUN、Crea水平高于非活动期LN组,C3、C4水平低于非活动期LN组(t=14. 21、17. 54、15. 45、17. 76、10. 76、12. 23、13. 56、14. 43,P <0. 05);非活动期LN组、活动期LN组血清ESR、Proteinuria、SLEDAI、水平高于对照组,活动期LN组血清ESR、Proteinuria、SLEDAI水平高于非活动期LN组(t=13. 87、15. 35、17. 54、18. 12、19. 09、13. 23,P <0. 05);血清和尿液IL-32和KIM-1水平与BUN、Crea、ESR、Proteinuria、RSLEDAI正相关关系明显,与C3、C4负相关关系明显(P <0. 05);尿液IL-32、血清IL-32、尿液KIM-1、血清KIM-1、Proteinuria均为LN患者处于活动期的危险因素(OR=17. 89、4. 26、5. 02、2. 43、13. 62,P <0. 05)。结论 LN患者血清和尿液中IL-32和KIM-1的表达水平升高,其与BUN、Crea、ESR、Proteinuria、C3、C4、SLEDAI相关关系明显;尿液和血清IL-32、KIM-1及Proteinuria均为LN患者处于活动期的危险因素。

Objective To investigate the expression and clinical significance of IL-32 and KIM-1 in serum and urine of patients with lupus nephritis(LN).Methods A total of 166 patients with LN who were treated in our hospital were divided into inactive LN group(n=86)and active LN group(n=80),moreover,the other 70 healthy subjects who underwent physical examination at the same period were enrolled as control group.The levels of IL-32 and KIM-1 were detected and compared among the three groups.Results The levels of IL-32 and KIM-1 in serum and urine in inactive LN group and active LN group were significantly higher than those in control group,and which in active LN group were significantly higher than those in inactive LN group(P<0.05).The serum levels of BUN and Crea in inactive LN group and active LN group were significantly higher than those in control group,however,the serum levels of C3,C4 were significantly lower than those in control group.Moreover the serum levels of BUN and Crea in active LN group were significantly higher than those in inactive LN group,but the levels of C3 and C4 in active LN group were significantly lower than those in inactive LN group(P<0.05).In addition the serum levels of ESR,proteinuria,SLEDAI in inactive LN group and active LN group were significantly higher than those in control group,which in active LN group were significantly higher than those in active LN group(P<0.05).Besides the levels of IL-32 and KIM-1 in serum and urine were positively correlated with those of BUN,Crea,ESR,proteinuria and RSLEDAI,however,which were negative correlated with those of C3 and C4(P<0.05).Furthermore of the urine IL-32,serum IL-32,urine KIM-1,serum KIM-1 and proteinuria were the risk factors in patients with active lupus nephritis(OR=17.89,4.26,5.02,2.43,13.62,P<0.05).Conclusion The expression levels of IL-32 and KIM-1 in serum and urine in patients with lupus nephritis are increased obviously,which are correlated with BUN,Crea,ESR,proteinuria,C3,C4,SLEDAI.Moreover urine IL-32,serum IL-32,urine KIM-1,serum KIM-1 and proteinuria are the risk factors in patients with active lupus nephritis.