基于基因芯片技术探究柴朴汤对哮喘大鼠差异表达基因的调控

To study the effect of Chaipu decoction on differentially expressed genes in asthmatic rats by gene chip

柴朴汤治疗哮喘的作用已经得到充分验证,但其作用机理尚不明确。既往研究表明,柴朴汤主要通过改善哮喘的气道炎症发挥其药理作用。本实验通过对哮喘模型大鼠及柴朴汤干预后的哮喘模型大鼠进行基因芯片分析,探测柴朴汤对炎性基因表达的影响。结果提示,T-淋巴细胞相关蛋白(MAL)及肿瘤蛋白D52L(TPD52L)基因与哮喘的发病呈显著相关性,柴朴汤则可以通过调节这两种差异性基因的表达参与哮喘的气道炎症及气道重塑。

Chaipu decoction can be used to treat asthma, but its mechanism of action is still unclear. Previous studies have shown that Chaipu decoction can improve airway inflammation in asthma, and this paper researches how it participates in inflammatory changes. In this study, the effects of Chaipu decoction on asthmatic rats were investigated by gene chip analysis of asthma model rats and asthmatic model rats after intervention with Chaipu decoction. The results showed that the expression of T-lymphocyte-associated protein (MAL) and tumor protein D52L (TPD52L) was significantly changed after Chaipu decoction (P<0. 05). Chaipu decoction may be involved in airway inflammation and airway remodeling in asthma by regulating the expression of these two differential genes.