四种常见离体脱位牙贮存介质对其牙周膜成纤维细胞生物活性的影响

Effect of Four Common in Vitro Dislocated Teeth Storage Medium on the Biological Activity of Periodontal Ligament Fibroblasts

目的:评价比较四种常见离体脱位牙贮存介质对牙周膜成纤维细胞活性的影响。方法:选取2017年1月-2018年1月本院口腔临床14～22岁青少年200例因正畸需要拔除的下颌第一前磨牙200颗作为研究对象,将其随机分为四组试验组(生理盐水组、牛奶组、唾液组、自来水组)和对照组,各40颗。培养脱位牙中的牙周膜成纤维细胞,其中试验分别采用生理盐水、牛奶、唾液、自来水作为贮存介质,对照组则直接在空气中保存。采用CCK-8法分别检测牙周膜细胞在四种常见离体脱位牙贮存介质中2、6、12、24 h不同时间点的活性,并与对照组进行比较。结果:各试验组不同时间点的牙周膜细胞存活率均明显高于对照组,差异均有统计学意义(P<0.05)。生理盐水、牛奶及唾液组不同时间点的牙周膜细胞存活率均明显高于自来水组,差异均有统计学意义(P<0.05)。牛奶、唾液组不同时间点的牙周膜细胞存活率均明显高于生理盐水,且牛奶组12、24 h的牙周膜细胞存活率均明显高于唾液组,差异均有统计学意义(P<0.05)。结论:牛奶、唾液贮存的牙周膜成纤维细胞活性较高,可作为有效的离体脱位牙临时贮存液,而生理盐水的保存效果次之,而自来水保存效果最差,不适合作为贮存介质。

Objective:To evaluate the effect of four common in vitro dislocated teeth storage medium on the biological activity of periodontal ligament fibroblasts.Method:A total of 200 mandibular first premolars extracted from 200 cases of 14-22 year old adolescents in our hospital due to orthodontic treatment from January 2017 to January 2018 were selected as the research objects.They were randomly divided into four experimental groups(saline group,milk group,saliva group,tap water group)and control group,40 teeth in each group.The periodontal ligament fibroblasts were cultured in the dislocation teeth.The experimental group were treated with saline,milk,saliva and tap water as storage medium respectively,while the control group was stored directly in air.The periodontal ligament cells were detected by CCK-8 method in the storage medium of four common in vitro dislocation teeth for 2,6,12 and 24 h respectively.The activity at different time points were compared with the control group.Result:The survival rate of periodontal ligament cells in each experimental group at different time points were significantly higher than those in the control group,the differences were statistically significant(P<0.05).The survival rate of periodontal ligament cells in the saline,milk and saliva groups at different time points were significantly higher than those in the tap water group,the differences were statistically significant(P<0.05).The survival rate of periodontal ligament cells in the milk and saliva groups at different time points were significantly higher than those in the saline group,and the survival rate of periodontal ligament cells in the milk group at 12 and 24 h was significantly higher than that in the saliva group,the differences were statistically significant(P<0.05).Conclusion:The periodontal ligament fibroblasts stored in milk and saliva have higher activity and can be used as effective temporary storage solution for dislocated teeth in vitro.The storage effect of saline is the second,while that of tap water is the worst,and it is not suitable for storage medium.