基于微流控技术的纳升级蛋白质结晶筛选方法的研究进展

Advances in Microfluidic Techniques for Nanoliter-Scale Protein Crystallization Screening

X射线晶体学技术是目前蛋白质结构测定中最主要的方法。为了得到满足衍射要求的高质量蛋白质晶体,研究者通常耗费大量试剂和样品进行大规模结晶条件筛选。微流控技术通过对超微量流体的操纵,可大幅降低在蛋白质结晶筛选中蛋白样品的消耗。本文依据结晶方法,分别介绍了基于微批量法、蒸气扩散法、自由界面扩散法和透析法的微流控蛋白质结晶筛选方法的研究进展。

X-ray crystallography is the major approach in the determination of protein structure.To obtain high quality protein crystals that meet the diffraction requirements,researchers usually spend large amounts of reagents and samples for large-scale crystallization condition screening.Microfluidic technology can greatly reduce the consumption of protein samples in protein crystallization screening by manipulating ultramicro amounts of fluids.In this review,the recent progress in microfluidic protein crystallization screening methods based on the microbatch,vapor diffusion,free-interface diffusion and dialysis methods is introduced according to the difference in crystallization methods.