淫羊藿苷对顺铂致卵巢早衰大鼠卵巢储备功能的作用研究

Effects of icariin on ovarian reserve function in female rats with premature ovarian failure induced by cisplatin

目的通过观察淫羊藿苷对顺铂腹腔化疗所致卵巢早衰大鼠卵巢卵泡数量和质量及卵巢颗粒细胞分泌抗苗勒氏管激素(AMH)的影响,从卵巢储备功能角度探讨淫羊藿苷治疗卵巢早衰的机制。方法采用随机数字表法将40只Wistar大鼠分为正常组、模型组、淫羊藿苷低剂量(25mg/kg)组和淫羊藿苷高剂量(75mg/kg)组4组,每组10只。实验开始日(第0天)予淫羊藿苷低剂量组淫羊藿苷25mg/kg(溶于0.5ml的0.9%氯化钠注射液中)腹腔注射给药,1次/3d,至第28天实验结束;淫羊藿苷高剂量组淫羊藿苷75mg/kg,其余方法同淫羊藿苷低剂量组。除空白组外,其余3组大鼠建立卵巢早衰动物模型,分别于第14、21天腹腔注射顺铂。第28天处死大鼠,采用ELISA法检测大鼠血清AMH水平,HE染色观察卵巢卵泡数量和形态的变化。结果与正常组大鼠比较,模型组大鼠血清AMH水平下降,卵巢卵泡数量减少,差异均有统计学意义(均P<0.05);与模型组大鼠比较,淫羊藿苷低剂量组和淫羊藿苷高剂量组大鼠血清AMH水平升高,卵巢卵泡数量增加,差异均有统计学意义(均P<0.05)。结论淫羊藿苷可能通过促进卵巢颗粒细胞AMH的分泌,恢复卵泡正常募集和成熟,从而保护卵巢储备功能。

Objective To investigate the effects of icariin(ICA)on ovarian reserve function in female rats with premature ovarian failure induced by cisplatin.Methods Forty healthy female Wistar rats were randomly divided into normal group,model group,low dose ICA group(25mg/kg),high dose ICA group(75mg/kg)with 10 rats in each group.The rat model of cisplatin-induced premature ovarian failure was established by intraperitoneal injection of cisplatin at d14 and d28.The intraperitoneal injection of ICA 25 or 75mg/kg was given every 3d starting from d0 till d28 in ICA treatment groups.Then the rats were sacrificed,the number and morphology of ovarian follicles were observed by HE staining and the serum AMH levels were measured by ELISA.Results Compared with the normal group,the ovarian follicular number in model group was reduced,and the serum AMH level in model group was decreased(both P<0.05).Compared with the model group,the ovarian follicular number and serumAMH levels were increased in the ICA treatment groups(all P<0.05).Conclusion ICA may protect the ovarian reserve function by promoting the secretion of AMH in ovarian granulosa cells and restoring the normal recruitment and maturation of ovarian follicles.