台湾相思内生真菌的分离筛选及其对幼苗生长的影响

Isolation and Screening of Endophytic Fungi from Acacia confuse and Their Effects on Seedling Growth

为促进沿海防护林树种台湾相思的生长发育,分离其内生真菌后筛选出强势菌株A(Trichoderma sp.Pd1)、B(Filobasidium sp. As3)、C(Fusarium sp. Pd2)、D(Colletotrichum sp. Ys6)、E(Fusarium sp. Pd4),并将不同菌株及菌株组合浇根回接于幼苗,测定其对台湾相思幼苗生长指标与叶绿素质量分数差异。结果表明:菌株AE、E、AD、CD和CE对植株苗高生长有极显著的促进作用;大部分菌株对植株地径生长均有促进作用,其中菌株AB、AE、DE、AD作用最为显著;菌株B和C极显著的增加了植株的生物量和根冠比;菌株CE在试验菌株中对台湾相思幼苗光合作用的影响最佳。总之,菌株A、C、E、AE、CD对台湾相思幼苗所测各项生理指标均有促进作用,AE对其促生效果最佳。

To promote the growth and development of the Acacia confusa Merr.in coastal protection forest tree species,the strong strains were screened out after isolating the endophytic fungus:A(Trichoderma sp.Pd1),B(Filobasidium sp.As3),C(Fusarium sp.Pd2),D(Colletotrichum sp.Ys6)and E(Fusarium sp.Pd4),and the root casted bacteria were returned to the seedlings to determine the difference between the growth index and the chlorophyll content of the A.confusa seedlings.The strains AE,E,AD,CD and CE significantly promoted the growth of plant seedlings;most of the strains had the promotion effect on the growth of plant ground diameter,of which the strain AB,AE,DE and AD were the most significant;the strain B and C significantly increased the plant biomass and root and crown ratio;strain CE in the experimental strains had the best effect on the photosynthesis of A.confusa seedlings.The strains A,C,E,AE and CD could promote the physiological indexes of A.confuse seedlings,and AE had the best promoting effect on them.