逆针“丰隆”穴对大鼠肥胖的预防作用及对转录因子过氧化物酶体增值物激活受体γ的影响

Effects of Preventive Acupuncture at the Fenglong Acupoint on Obesity Prevention and PPAR-γ Expression Level in Rats Fed a High-fat Diet

背景目前疾病医学逐渐出现向健康医学转变的趋势,医学干预的切入点逐渐提前。"治未病"是中医预防医学思想的重要体现,而逆针灸是其重要手段之一。目的观察逆针"丰隆"穴对大鼠肥胖的预防作用及对转录因子过氧化物酶体增值物激活受体γ(PPAR-γ)的影响。方法研究时间为2016年5—7月。将4周龄SPF级雄性大鼠24只随机分为空白组、模型组和逆针组,每组8只。模型组和逆针组进行高脂饲料喂养,空白组进行普通饲料喂养,为期12周,造模同时进行针刺干预,逆针组大鼠固定在自制固定器上,待大鼠平静后,逆针双侧"丰隆"穴区域每周3次,空白组和模型组同步抓取固定,不干预。干预期间每周测量并记录各组大鼠体质量,干预12周后检测各组大鼠肾周脂肪组织重量、脂肪细胞形态学、血清瘦素水平及脂肪组织中转录因子PPAR-γ表达水平。结果干预第8周,模型组大鼠体质量高于空白组(P<0.05);干预第12周,模型组、逆针组大鼠体质量高于空白组(P<0.05),逆针组大鼠体质量小于模型组(P<0.05)。模型组、逆针组大鼠肾周脂肪组织重量大于空白组(P<0.05);逆针组大鼠肾周脂肪组织重量低于模型组(P<0.05)。模型组大鼠脂肪细胞面积较空白组大鼠明显增大,新生脂肪细胞较多;逆针组大鼠脂肪细胞较模型组明显面积减小,新生脂肪细胞较少;模型组大鼠脂肪细胞平均面积大于空白组、逆针组(P<0.05)。模型组大鼠血清瘦素水平高于空白组(P<0.01);逆针组大鼠血清瘦素水平低于模型组(P<0.01)。模型组大鼠脂肪组织中转录因子PPAR-γ表达水平高于空白组(P<0.05);逆针组大鼠脂肪组织中转录因子PPAR-γ表达水平低于模型组(P<0.05)。结论逆针"丰隆"穴可以有效预防大鼠肥胖,改善脂肪代谢的异常状态,其机制可能与改善瘦素抵抗及调节脂肪组织中PPAR-γ的表达有关。

Background At present,disease medicine is gradually changing to health medicine,and the time point of medical intervention is gradually getting earlier.Preventive treatment of disease is an important manifestation of TCM preventive medicine,and preventive acupuncture and moxibustion is one of its important means.Objective To observe the effect of preventive acupuncture at the Fenglong acupoint on obesity prevention and PPAR-γexpression level in rats fed a high-fat diet.Methods This study was implemented during May to July 2016.Twenty-four four-week-old male SPF male rats were randomly divided into blank group,model group and preventive acupuncture group,with eight in each.All groups received a 12-week intervention,during this period,the blank group received a normal diet plus being taken and fixed at the self-made fixator(three times a week),the model group received a high-fat diet plus being taken and fixed at the self-made fixator(three times a week),and the preventive acupuncture group received a high-fat diet plus preventive acupuncture treatment(bilateral needling of the Fenglong acupoint after being fixed at the self-made fixator,three times a week);body weight was measured once a week.When the intervention ended,the rats were scarified and perirenal fat weight was measured,morphology of the adipocyte area was observed,serum leptin and PPAR-γexpression levels in the adipose tissue were measured.Results At the end of the 8th week of intervention,the blank group showed much lower body weight than the model group(P<0.05).At the end of the intervention,the body weight was much lower in the blank group than that of the model group and preventive acupuncture group(P<0.05);the preventive acupuncture group demonstrated significantly decreased body weight compared with the model group(P<0.05).The blank group exhibited significantly decreased perirenal fat weight compared with other two groups(P<0.05).The perirenal fat weight of the preventive acupuncture group was much lower than that of the model group(P<0.05).Compared with the model group,both the blank group and preventive acupuncture group showed much less newly generated adipocytes and much smaller adipocyte areas.The average area of adipocytes in model group was larger than that in blank group and preventive acupuncture group(P<0.05).The model group had significantly higher serum leptin level compared with other two group(s P<0.01).The PPAR-γexpression level in the adipose tissue in the model group was obviously higher than that of the blank group and preventive acupuncture group(P<0.05).Conclusion Preventive acupuncture at the Fenglong acupoint can effectively prevent obesity in rats and improve the abnormal state of fat metabolism.The mechanism may be related to the improvement of leptin resistance and the regulation of PPAR-γexpression level in the adipose tissue.