不同浓度氟化钠抗凝血对POCT血糖仪校准结果干扰影响的研究

Anticoagulant Blood of Different Sodium Fluoride Concentration Interfered Calibrated Results of POCT Glucometer

目的研究不同浓度氟化钠抗凝血对POCT血糖仪结果干扰情况。方法 20例研究对象每人采集1支EDTA-K2样本管和6支含不同浓度氟化钠的(NaF)EDTA-K2样本管静脉血,每管2ml,POCT血糖仪与生化仪检测每管血糖水平,比较不同浓度氟化钠组间样本管血糖水平差异及相同氟化钠浓度样本管POCT血糖仪与生化仪检测血糖水平差异。结果 POCT血糖仪检测不同浓度氟化钠样本管血糖水平组间比较,其差异有统计学意义(F=12.192,P<0.001);采用LSD法进行多重比较,不含NaF组血糖水平与其它含NaF组血糖水平比较,不含NaF组血糖水平明显高于其它含NaF组血糖水平(t=13.556,14.223,13.512,14.012,13.876,13.864,均P<0.001),含NaF不同组间血糖水平比较,差异无统计学意义(t=0.352,0.412,0.386,0.411,0.395,0.414,均P>0.05);生化仪检测不同浓度氟化钠组间血糖水平比较,其差异无统计学意义(F=0.428,P=0.829)。不含氟化钠样本管两仪器检测血糖水平比较,差异无统计学意义(t=0.457,P=0.358);含相同浓度氟化钠样本管两仪器检测血糖水平比较,生化仪检测血糖水平明显高于POCT血糖仪水平(t=-14.415,-14.746,-13.613,-14.108,-15.253,-14.069,均P<0.01)。结论氟化钠对采用葡萄糖脱氢酶法POCT血糖仪结果有明显抑制作用,抑制程度与氟化钠浓度无明显相关,氟化钠不宜作为葡萄糖脱氢酶法POCT血糖仪比对标本的糖酵解抑制剂。

Objective To study calibrated results of POCT glucometer that were interfered different concentration sodium fluoride anticoagulant blood.Methods This study recruited 20 healthy volunteers.Each of healthy volunteer was collected seven venous blood sample tubes including one tube containing dipotassium ethylene diamine tetraacetate(EDTA-K2)and six tubes containing different concentration of sodium fluoride and dipotassium ethylene diamine tetraacetate(NaF/EDTA-K2),2 ml per tube.The blood glucose levels of all tubes were detected by POCT glucometer and biochemical analyzer.The blood glucose levels of difference sodium fluoride concentrations group were compared.The difference of blood glucose levels of same sodium fluoride concentrations were compared between sodium fluoride concentrations group were compared.Results The blood glucose levels group detected by POCT glucometer were compared of different sodium fluoride concentrations,and the difference was statistically significant(F=12.192,P<0.001).The blood glucose levels of content no sodium fluoride was higher than the blood glucose levels of others content sodium fluoride(t=13.556,14.223,13.512,14.012,13.876,13.864,all P<0.001)and the blood glucose levels of content different sodium fluoride concentrations group were compared.The difference was not statistically significant(t=0.352,0.412,0.386,0.411,0.395,0.414,all P >0.05)by LSD multiple comparisons of one-way ANOVA.The blood glucose levels detected by biochemical analyzer were compared with differenct sodium fluoride concentrations group,the difference was not statistically significant(F=0.428,P=0.829).The blood glucose levels of content no sodium fluoride compared between POCT glucometer and biochemical analyzer,the difference was not statistically significant(t=0.457,P=0.358).The blood glucose levels of content same sodium fluoride concentrations compared between POCT glucometer and biochemical analyzer.The blood glucose levels of biochemical analyzer were higher than POCT glucometer(t=-14.415,-14.746,-13.613,-14.108,-15.253,-14.069,all P<0.01).Conclusion Sodium fluoride inhibited blood glucose results of POCT glucometer by enzymatic glucose dehydrogenation method.Inhibition degree was not associated with content of sodium fluoride significantly.The comparison specimen of POCT glucometer by enzymatic glucose dehydrogenation should not use sodium fluoride as glycolysis inhibitors.