溶血磷脂酰胆碱衍生物的合成及其对自然杀伤T细胞的激活作用

Synthesis of Immunostimulant Lysophosphatidylcholine Analogues and Their Agonist Activities for Natural Killer T Cells

溶血磷脂酰乙醇胺(pLPE)是自然杀伤T细胞(NKT)的内源性抗原。溶血磷脂酰胆碱衍生物(LPC)与CD1d结合被NKT细胞识别并激活NKT细胞。本文以手性甘油醇缩丙酮为起始原料,合成了LPC衍生物(5和6),其结构经~1H NMR,^(13)C NMR,^(31)P NMR和HR-MS(ESI)确证。研究了5和6的免疫活性。结果表明:100 ng·mL^(-1)6负载mCD1d分子后可刺激2H4细胞释放IL-2因子(32.13±1.56 pg·mL^(-1)),具有潜在的NKT激活性质。

Plasmalogen lysophosphatidylethanolamines(pLPE),had been found as self antigens for natural killer T cells(NKT cells).Lysophosphatidylcholines(LPC)was reported to be recognized by NKT cells as antigens presented by CD1d.Two LPC derivatives(5 and 6)were synthesized using 2,3-O-isopropylidene-sn-glycerol as material.The structures were confirmed by^1 H NMR,^13 C NMR,^31 P NMR and HR-MS(ESI).The immunocompetence of 5 and 6 were investigated.The results showed that 100 ng·mL^-1 6 loaded with plate-bound mCD1d stimulated NKT 2H4 cells to release IL-2(32.13±1.56 pg·mL^-1).The compound 6 was a potential new NKT agonist.