温阳振衰颗粒对心肌细胞损伤模型LncRNABIC的影响

Effects of Wenyang Zhenshuai Granule on LncRNA BIC in Myocardial Cell Injury Model

目的观察温阳振衰颗粒含药血清对H9C2心肌细胞损伤模型LncRNA BIC表达的影响并探讨其作用机制。方法 H9C2心肌细胞分为正常对照组、正常加含药血清组、阿霉素(ADR)组(ADR组)、ADR加含药血清组。其中ADR组、含药血清组分别为在培养基中加入2.67μmol/L的ADR和加入10%的温阳振衰颗粒含药血清。各组均培养相同时间后检测实验末各组细胞LncRNA BIC的表达水平。结果倒置显微镜下可见正常对照组、正常加含药血清组心肌细胞形态、胞质、间质及横纹均为正常;ADR组心肌细胞出现肌纤维的溶解,横纹模糊,及胞浆空包变性,且表现出不同程度的炎症细胞浸润和间质水肿。ADR加含药血清组的心肌细胞较ADR组,其肌纤维溶解、恒温模糊、胞浆空泡变性、炎症细胞浸润、间质水肿等情况均有所改善。正常对照组、正常加含药血清组的细胞存活率相当(P> 0.05);ADR组、ADR加含药血清组与正常对照组比较,其细胞存活率均明显下降(P <0.05);ADR加含药血清组与ADR组比较,其细胞存活率有所上升(P <0.05)。正常加含药血清组与正常组比较,LncRNA BIC表达明显升高(P <0.05);ADR组与正常组比较,LncRNA BIC表达明显降低(P <0.05);ADR加含药血清组与ADR组比较,LncRNA BIC表达升高(P <0.05)。结论温阳振衰颗粒能上调LncRNA BIC的表达,这可能是其治疗慢性心衰的重要机制之一。

Objective:To observe the effects of Wenyang Zhenshuai Granule containing serum on the expression of LncRNA BIC in H9C2 myocardial cell injury model.Methods:H9C2 myocardial cells were divided into the normal control group,normal+drug serum group,ADR group,and ADR+drug serum group.2.67μmol/L ADR and 10%drug serum were added to ADR group and ADR+drug serum group,respectively.LncRNA BIC expression in these groups were detected by RT-PCR.Results:Compared with the normal control group,the expression of LncRNA BIC was significantly down-regulated in ADR group;the difference was statistically significant(P<0.05).Drug serum group could significantly increase the expression of LncRNA BIC;the difference was statistically significant(P<0.05).Conclusion:Wenyang Zhenshuai Granule can up-regulate the expression of LncRNA BIC,which may be one of the important mechanisms on chronic heart failure.