不同培养基对春小麦×玉米单倍体胚分化成苗的影响

Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

【目的】筛选适宜春小麦单倍体胚分化成苗的培养基,获得稳定且高效的单倍体胚培养方法。【方法】分别使用8种不同配方培养基对春小麦单倍体胚进行离体培养,使其分化成苗,根据成苗率及生长情况筛选培养基。【结果】在不添加激素的1/2 MS培养基中春小麦单倍体胚成苗率最高(73.49%),在1/2MS+0.1 mg/L 2,4-D培养基中春小麦单倍体胚能够一步成苗,且生长健壮,根系发达。【结论】1/2 MS培养基较B5培养基更适宜春小麦单倍体胚的分化成苗,2,4-D对于春小麦单倍体胚的根系发育具有明显的促进作用,2,4-D>IAA,1/2 MS+0.1 mg/L 2,4-D培养基是春小麦单倍体胚分化成苗的适宜培养基。

【Objective】This project aims to screen suitable medium for differentiation of spring wheat haploid embryos and obtain a stable and efficient method.【Method】The haploid embryos were differentiated into plantlets using 8 different mediums,and the mediums was screened according to the seedling rate and growth conditions.【Result】The seedling rate of spring wheat haploid embryos was the highest(73.49%)in 1/2 MS medium without hormones.The haploid embryos were induced one-step-seedling in 1/2 MS+0.1 mg/L 2,4-D medium,with stronger plantlets and their roots developed well.【Conclusion】The 1/2 MS medium was more suitable for the differentiation of spring wheat haploid embryos than the B5 medium,and the 2,4-D had more significant effect on the development of roots and differentiation of embryos.The medium of 1/2 MS+0.1 mg/L 2,4-D was a suitable medium for haploid embryos differentiation of spring wheat.