B7-H3在肺癌恶性胸腔积液中的表达及临床意义

Detection of B7-H3 in pleural effusion of patients with lung cancer and its clinical significance

目的探讨B7-H3在肺癌患者胸腔积液中的表达水平及临床意义。方法选取66例胸腔积液患者为研究对象,33例为肺癌合并恶性胸腔积液患者,其余33例为非恶性胸腔积液患者。采用酶联免疫吸附法(ELISA)检测两组患者胸腔积液、外周血B7-H3水平;采用流式细胞术(FCM)检测两组患者胸腔积液、外周血CD_4^+CD_(25)^+调节性T细胞(CD_4^+CD_(25)^+Treg)比例。结果肺癌患者胸腔积液B7-H3含量、CD_4^+CD_(25)^+Treg比例明显高于其外周血(P <0. 05);恶性胸腔积液中B7-H3含量、CD_4^+CD_(25)^+Treg比例明显高于非恶性胸腔积液(P <0. 05)。结论胸腔积液B7-H3的表达水平对良恶性胸水的鉴别具有重要的临床意义。

Objective To explore the level of B7-H3 in pleural effusion of patients with lung cancer and elucidate its clinical significance. Methods 66 patients with pleural effusions were selected,including 33 cases of malignant pleural effusion and 33 cases of benign pleural effusion. The expression of B7-H3 in pleural effusion and peripheral blood were tested by enzyme linked immunosorbent assay( ELISA) technique. Flow cytometry( FCM) was used to detect the percentages of CD4+CD25+ regulatory T cells( Treg) of pleural effusion and peripheral blood. Results The levels of B7-H3 and CD4+CD25+ Treg percentage in pleural effusion of lung cancer patients were significantly higher than those in peripheral blood( P < 0. 05). The levels of B7-H3 and CD4+CD25+ Treg percentage in pleural effusion of lung cancer patients were significantly higher than those in benign pleural( P < 0. 05). Conclusion The detection of pleural effusion B7-H3 has the significance of differential diagnosis between benign and malignant pleural effusion.