HPLC法同时测定牛黄清感胶囊中8种有效成分的含量

Simultaneous Determination of Eight Active Ingredients in Niuhuang Qinggan Capsules by HPLC Method

[目的]建立牛黄清感胶囊HPLC含量测定,用于该制剂的质量控制。[方法]运用Waters Alliance 2695高效液相色谱仪,采用C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.5%磷酸溶液,梯度洗脱,柱温40℃,流速为1.0 mL/min,检测波长为350 nm。[结果]建立同时测定牛黄清感胶囊8个成分隐绿原酸,绿原酸,异绿原酸C,连翘酯苷A,黄芩苷,黄芩素,汉黄芩苷和汉黄芩素的方法。[结论]该方法稳定、准确、重现性好,专属性强,可用于牛黄清感胶囊的质量控制。

[Objective]To establish HPLC determination of Niuhuang Qinggan capsule for quality control of the preparation.[Methods]Waters Alliance 2695 HPLC is used on a C18 column(4.6 mm×250 mm,5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid solution.Elution with gradient,column temperature is 40°,the flow rate is 1.0 mL/min,detection wavelength is 350 nm.[Reslts]A method was established for the simultaneous determination of cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid C,forsythiaside A,baicalin,baicalein,wogonin and wogonin in 8 components of Niuhuang Qinggan Capsules.[Conclusion]This method is stable,accurate,reproducible,and highly specific.It can be used for the quality control of Niu Huang Qinggan capsules.