摘要
Fruit of Lonicera caerulea L. is a rich source of phenolic compounds such as phenolic acids as well as anthocyanins,proanthocyanidins and other flavonoids,which display potential health-promoting effects. However,its genetics and genomic information is limited. Transcriptome and digital gene expression( DGE) profiles of fruit at four different development stages were compared. Related genes with anthocyanin biosynthesis were compared using real-time quantitative PCR( qRT-PCR). RNA-Seq generated 6. 26-Gb clean reads,which was then de novo assembled into 45,656 UniG enes with a mean length 837 bp. A total of 7 928 unigenes were annotated into 32 known metabolic or signaling pathways in which a few primary,intermediate,and secondary metabolic pathways are directly related to fruit quality. The result of DGE and qRT-PCR indicates that UFGT( comp20132_c0) may play a key role in honeysuckle coloration. This provides a reference for the study of complicated metabolism in non-model perennial species.
Fruit of Lonicera caerulea L. is a rich source of phenolic compounds such as phenolic acids as well as anthocyanins,proanthocyanidins and other flavonoids,which display potential health-promoting effects. However,its genetics and genomic information is limited. Transcriptome and digital gene expression( DGE) profiles of fruit at four different development stages were compared. Related genes with anthocyanin biosynthesis were compared using real-time quantitative PCR( qRT-PCR). RNA-Seq generated 6. 26-Gb clean reads,which was then de novo assembled into 45,656 UniG enes with a mean length 837 bp. A total of 7 928 unigenes were annotated into 32 known metabolic or signaling pathways in which a few primary,intermediate,and secondary metabolic pathways are directly related to fruit quality. The result of DGE and qRT-PCR indicates that UFGT( comp20132_c0) may play a key role in honeysuckle coloration. This provides a reference for the study of complicated metabolism in non-model perennial species.
基金
Supported by Agricultural Science and Technology Innovation Project of China
