摘要
[Objectives]This study was conducted to study the Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi under osmotic stress.[Methods]The activity of praline,soluble sugar and protein was detected with ultraviolet spectrophotometry.The activity of phenylalanine ammonia-lyase(PAL)and cinnamic acid 4-hydroxylase(C_4H)was determined with ultraviolet spectrophotometry.The secondary metabolites were detected by high performance liquid chromatography(HPLC).[Results]The germination percentage,germination potentiality and germination index decreased at first and increased subsequently along with the enhancement of PEG concentration.The praline content,the soluble sugar content and the soluble protein content in S.baicalensis seedlings increased along with the enhancement of PEG concentration and the differences from the CK were significant(P<0.05).The PAL and C_4H in S.baicalensis seedlings decreased after osmotic stress and the differences from the CK were significant(P<0.05).The results indicate that,the scutellarin,baicalin,wogonoside,baicalein,wogonin and oroxylinA contents decreased after osmotic stress and the differences from the CK were significant(P<0.05).[Conclusions]The quality of the skullcaps could be improved by improving the activity of PAL and C_4H.
[Objectives]This study was conducted to study the Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi under osmotic stress.[Methods]The activity of praline,soluble sugar and protein was detected with ultraviolet spectrophotometry.The activity of phenylalanine ammonia-lyase(PAL)and cinnamic acid 4-hydroxylase(C_4H)was determined with ultraviolet spectrophotometry.The secondary metabolites were detected by high performance liquid chromatography(HPLC).[Results]The germination percentage,germination potentiality and germination index decreased at first and increased subsequently along with the enhancement of PEG concentration.The praline content,the soluble sugar content and the soluble protein content in S.baicalensis seedlings increased along with the enhancement of PEG concentration and the differences from the CK were significant(P<0.05).The PAL and C_4H in S.baicalensis seedlings decreased after osmotic stress and the differences from the CK were significant(P<0.05).The results indicate that,the scutellarin,baicalin,wogonoside,baicalein,wogonin and oroxylinA contents decreased after osmotic stress and the differences from the CK were significant(P<0.05).[Conclusions]The quality of the skullcaps could be improved by improving the activity of PAL and C_4H.
基金
Supported by 2017 Traditional Chinese Medicine Public Health Service Specific Grant(CS[2017]66)
Shandong Traditional Chinese Medicine Science and Technology Development Program(2017-522)
