羊骨胶原肽对去卵巢大鼠的骨质改善作用

Sheep bone collagen peptide improve bone quality of ovariectomized rats

[目的]探讨羊骨胶原肽(collagen peptide,CP)对去卵巢大鼠的骨质改善作用,为畜骨的高值利用和骨质疏松非药物防治的研发提供试验依据。[方法]切除大鼠双侧卵巢,设置切卵巢组(OVX)、雌激素组(E_2)、羊骨胶原肽的高、中、低剂量组(CP-H、CP-M、CP-L),另设假手术组(SHAM)。各组8只,饲喂基础日粮,CP各剂量组灌胃相应受试物,SHAM组和OVX组灌胃等体积蒸馏水,E_2组注射雌激素。灌胃9 w后处死大鼠,取股骨进行骨密度、骨矿物质、羟脯氨酸含量测定,并测定血清PINP及β-CTx水平。[结果]OVX组骨密度((1.767±0.031) g·cm^(-3))、骨矿物质(Ca、Mg、Zn、Mn)((9.73±0.14) mg·g^(-1),(0.34±0.01) mg·g^(-1),(30.48±1.48)μg·g^(-1),(1.70±0.24)μg·g^(-1))以及羟脯氨酸含量((1.922±0.12)μg·mg^(-1))均显著低于SHAM组((2.283±0.47) g·cm^(-3),(12.59±2.78) mg·g^(-1),(0.49±0.10) mg·g^(-1),(33.78±0.43)μg·g^(-1),(1.94±0.29)μg·g^(-1),(2.258±0.22)μg·mg^(-1)),血液PINP((3 528.37±154.74) pg·mL^(-1))和β-CTx((529.67±23.63) pg·mL^(-1))水平显著高于SHAM组((2.283±0.47) g·cm^(-3),(2 258.55±140.87) pg·mL^(-1),(370.98±61.89) pg·mL^(-1)),说明骨质疏松模型建模成功,CP各剂量组和E_2组骨密度、所测骨矿物质含量、羟脯氨酸含量均显著高于OVX组,血清PINP及β-CTx水平显著低于OVX组(P<0.05)。[结论]羊骨胶原肽可提高去卵巢大鼠股骨密度,抑制骨矿物质和骨有机质的丢失,降低血清骨代谢标志物水平,从而具有骨质改善作用。

[Objective]The positive effect of sheep bone collagen peptide(CP)on bone quality of ovariectomized rats was investigated to provide solid evidence for adding-value utilization of animal bone resources and non-medicine prevention of osteoporosis.[Methods]Bilateral ovariectomized rats were divided into six groups,including ovariectomized(OVX),estrogen(E2),CP at high level(CP-H),CP at medium level(CP-M),CP at low level(CP-L groups),and SHAM group which were sham operated.Eight rats were involved in each group.In addition to basal diet for all groups,CP groups were also intragastrically administered with CP at different dosages,while SHAM and OVX group were treated with equal-volume distilled water,and rats in E2 group were injected with 17-βestrodial.After nine weeks treatment,femoral bones of rats in each group were sampled to determine the bone density,bone mineral,and hydroxyproline content,and blood samples were collected to determine serum PINP andβ-CTx levels.[Results]Results showed that the bone density,bone minerals(Ca,Mg,Zn,Mn),and hydroxyproline content of OVX group((1.767±0.031)g·cm^-3,(9.73±0.14)mg·g^-1,(0.34±0.01)mg·g^-1,(30.48±1.48)μg·g^-1,(1.70±0.24)μg·g^-1,and(1.922±0.12)μg·mg^-1,respectively)were significantly lower than those of SHAM group((2.283±0.47)g·cm-3,(12.59±2.78)mg·g^-1,(0.49±0.10)mg·g^-1,(33.78±0.43)μg·g^-1,(1.94±0.29)μg·g^-1,and(2.258±0.22)μg·mg^-1,respectively).The serum PINP andβ-CTx levels of OVX at(3528.37±154.74)and(529.67±23.63)pg·mL^-1 were significantly higher than those in SHAM group at(2258.55±140.87)and(370.98±61.89)pg·mL^-1(P<0.05),which indicated that osteoporosis model was successfully established.Bone density,bone mineral,and hydroxyproline content in both CP groups and E2 group were significantly greater than those in the OVX group,while serum PINP andβ-CTx levels were significantly lower than OVX group(P<0.05).[Conclusion]Sheep bone collagen peptide can inhibit the loss of bone mineral and bone organic matter in ovariectomized rats and can be an important role in bone quality improvement.