基于液质联用技术的慢性肾脏病不同阶段患者血浆代谢组学研究

Study on plasma metabonomics of patients with different stages of chronic kidney disease based on HPLC-Q/TOF technology

目的对慢性肾脏病(CKD)不同阶段患者血浆差异化合物进行分析,探讨CKD发病机制,为前期诊断提供依据。方法采用液质联用技术的代谢组学方法,绘制CKDⅠ～Ⅲ、Ⅳ、Ⅴ期患者及健康对照者血浆代谢轮廓谱,利用Agilent MPP 12.1软件进行单因素方差分析、倍数检验及主成分分析等数据模式识别,寻找各组中血浆差异化合物,并对差异化合物进行结构鉴定与代谢通路分析。结果多变量统计分析结果显示,不同阶段CKD患者血浆标本存在明显差异,在血浆中找到鞘氨醇、牛磺酸、原卟啉Ⅸ等27个差异化合物。结论不同阶段CKD患者血浆中胆红素循环、鞘脂类代谢、类固醇类代谢等通路出现紊乱,该研究为CKD的临床诊断与治疗提供参考。

Objective To analyze the plasma differential components in the patients with different stages of chronic kidney disease(CKD),and to probe the pathogenesis of CKD in order to provide a basis for previous diagnosis.Methods The metabonomic method based on HPLC-Q/TOF technology was used to draw the plasma metabolic profiles in the patients with CKD stageⅠ-Ⅲ,ⅣandⅤand healthy controls.The data mode recognition of single factor analysis of variance,multiple testing and principal component were performed by using the Agilent MPP 12.1 software.The plasma differential compounds in each group were sought.The structures of different compounds were identified and the metabolic pathways were analyzed.Results The multivariate statistical analysis results showed that the plasma samples in the patients with different stages of CKD had obvious difference,and 27 different compounds in the plasma such as sphingosine,taurine,protoporphyrinⅨwere found.Conclusion The metabolic pathways of bilirubin circulation,sheath lipid metabolism and steroid metabolism in plasma of the patients with different stages of CKD appear the disorder,this study provides reference for the clinical diagnosis and treatment of CKD.