miR-1对HR HPV 16^+/18^+宫颈癌细胞周期相关蛋白的调控

The Expressional Regulation Effects of miR-1 on Cell-cycle Associated Proteins in High-risk(HR) Human Papillomavirus(HPV) Infected Cervical Cancer Cells

目的比较miR-1过表达及表达抑制后人宫颈癌细胞HPV16+Siha以及HPV18+Hela中细胞周期蛋白的表达影响,了解miR-1在HR HPV致瘤过程中的分子机制。方法构建miR-1过表达慢病毒载体及miR-1缺陷表达载体,转染高危型(HR) HPV16+/18+人宫颈癌细胞,运用Western blot方法对各组转染细胞中细胞周期蛋白Cyclin D1、Cyclin D2、Cyclin E的表达进行检测。结果 (1)成功获得miR-1过表达及表达抑制的人宫颈癌细胞株Hela和Siha;(2)与空白对照组相比,miR-1过表达及表达抑制后HR HPV16+/18+人宫颈癌细胞株中细胞周期蛋白出现了相应的表达改变。结论 miR-1在HR HPV+人宫颈癌中的致瘤性与其对细胞周期蛋白的表达调控密切相关。

Objective To explore the expressional changes of cell-cycle associated proteins,Cyclin D1,Cyclin D2 and Cyclin E,in high-risk(HR)human papillomavirus(HPV)infected(+)cervical cancer cells following miR-1 overexpression or inhibition.Method The lentiviral system and specific sponge target miR-1 were used to up-regulate or inhibit the expression of miR-1 in HR HPV16+Siha and HPV18+Hela cells.Western bot was employed to determine the expressional changes of Cyclin D1,Cyclin D2 and Cyclin E amang those differently treated cell lines.Results The expression of miR-1 in HR HPV16+Siha and HPV18+Hela cells were significantly up-regulated or inhibited after lentiviral system or sponge transfection.Western blot analysis revealed that the relative expressions of Cyclin D1,Cyclin D2 and Cyclin E in HR HPV+Hela/Siha cells were altered,following miR-1 overexpression or inhibition.Conclusion The expressional changes of various cell cycle related proteins were associated with the oncogenesis events of miR-1 in HR HPV infected cervical cancer cells.