激素和光照对青蒿素合成途径酶基因表达的调控研究

Regulation of the Expression of the Genes on Artemisinin Synthesis Pathway by Hormone and Light

青蒿素是治疗疟疾的有效成分,其来源主要是从青蒿地上部分提取而来,但青蒿中青蒿素的含量确却低仅为干重的0.1%～0.8%,因此提高青蒿中青蒿素的含量具有重要的研究意义。青蒿素作为次生代谢产物,其合成受到外界环境的影响。通过施加水杨酸(SA)、甲基茉莉酸(MeJA)、赤霉素(GA)、脱落酸(ABA)、生长素(IAA)以及红光等不同处理,模拟外界环境的影响,分析青蒿素合成途径关键酶基因表达水平的变化,从而为提高青蒿中青蒿素的含量打下基础。

Artemisinin is an effective component in the treatment of malaria which is mainly extracted from the aerial part of Artemisia annua,however,the content of artemisinin in Artemisia annua is extremely low,only 0.1–0.8% of dry weight.Therefore,it is very important to study how to improve the content of artemisinin in Artemisia annua.As a secondary metabolite,the synthesis of artemisinin is affected by environment.Through applying salicylic acid(SA),methyl jasmonic acid(MeJA),gibberellin(GA),abscisic acid(ABA),auxin(IAA)and red light to mimic the effects of environment on expression of the genes on artemisinin synthesis pathway will lay the foundation for improving the content of artemisinin in Artemisia annua.