棕榈蓟马2种杀虫剂抗感种群间多种酶活性的比较

Comparative studies on enzyme activity between resistant and susceptible Thrips palmi populations to insecticide

为明确乙基多杀菌素和吡虫啉对棕榈蓟马的抗性风险,采用叶管药膜法测定了棕榈蓟马种群对乙基多杀菌素和吡虫啉的抗性倍数,比较了其抗性种群和敏感种群之间羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GST)、乙酰胆碱酯酶(AchE)3种酶的活性和细胞色素P450、细胞色素b5的含量变化。结果表明,棕榈蓟马种群对吡虫啉产生了中等水平的抗性(抗性倍数为12.73),对乙基多杀菌素产生了低水平的抗性(抗性倍数为9.53)。除棕榈蓟马吡虫啉抗性种群(BK)的AchE活性下降(0.58倍)外,乙基多杀菌素抗性种群(DK)的AchE活性则提高为1.31倍;DK和BK的CarE活性分别提高为敏感种群的1.46、1.79倍,GST活性分别提高为1.40倍和1.59倍,细胞色素P450含量分别提高为2.48倍和4.25倍,细胞色素b5含量分别提高为1.79倍和3.53倍,均高于敏感品系酶活水平。其结果表明,棕榈蓟马体内酶活的变化是其对杀虫剂产生抗药性的重要因素。

Comparative study on activity of CarE,GST,AchE and content difference of cytochrome P450 and cytochrome b5 in the three enzymes in both susceptible and resistant population of Thrìps pa1mi were conducted through leaf tube residue method to discover resistance multiple of T.pa1mi against spinetoram and imidacloprid.The results showed that T.pa1mi had medium level of resistance to imidacloprid and low level to spinetoram,with the value of 12.73 and 9.53 respectively.As for enzyme activities of T.p a1mi population with resistance to imidacloprid(BK)and spinetoram(DK)compared with susceptible populations,only enzyrne activity of AchE of BK decreased to 0.58 times,while that of DK increased to 1.31 times;enzyme activity of CarE of DK and BK increased to 1.46 and 1.79 times,and that of GST increased to 1.40 and 1.59 times,repectively.Contents of cytochrome P450 of DK and BK increased to 2.48 and 4.25 times,and that of cytochrome b5 increased to 1.79 and 3.53 times,repectively.It indicated出at changes of en巧rne activity in T.p a1mi were the critical factor ofìncreasing insecticide resistance.