双硫仑联合铜对肺癌脑转移癌细胞增殖和迁移的影响

Effect of Disulfiram Combined with Copper on the Proliferation and Migration of Brain Metastases from Lung Cancer

目的探讨双硫仑联合铜(DSF/Cu)对肺癌脑转移癌细胞的增殖和迁移的影响。方法细胞分为空白对照组、丙戊酸钠(VPA)组(VPA 1.0 mmol·L-1)、DSF/Cu组(1,2,3,4,5 mg/0.17 mg),与肺腺癌脑转移癌细胞共同孵育72 h后,CCK-8法检测癌细胞增殖能力的变化、Transwell小室检测DSF/Cu对癌细胞迁移能力的影响、Annexin V-PI双染色法检测癌细胞凋亡程度。结果与空白对照组比较,各个剂量组肺腺癌脑转移癌细胞的增殖能力明显受到抑制,细胞的迁移能力显著降低,细胞凋亡率明显升高;随着DSF/Cu剂量增加,其抑制转移癌细胞增殖和迁移的能力、诱导转移癌细胞凋亡的能力逐渐增强。结论 DSF/Cu能抑制肺腺癌脑转移癌细胞的增殖和迁移,诱导细胞凋亡,双硫仑有望成为联合治疗脑转移癌的新手段。

Objective To investigate the effects of disulfiram combined with copper(DSF/Cu)on proliferation and migration of brain metastases from lung cancer.Methods The cell were divided into blank control group,valproate group(1.0 mmol·L^-1 of valproate),DSF/Cu(1,2,3,4,5 mg/0.17 mg).Brain metastases from lung adenocarcinoma were incubated 72 hours.The changes of proliferative ability of cancer cells were detected by CCK-8 method,the effect of DSF/Cu on the migration ability of cancer cells was detected by Transwell chamber,and the degree of apoptosis of cancer cells was detected by Annexus V-PI double staining method.Results The ability of proliferation was significantly inhibited,the ability of migration was significantly reduced,and the rate of cell apoptosis was significantly increased in each dose group of brain metastases from lung adenocarcinoma compared with the blank control group.With the increase of DSF/Cu dose,its ability to inhibit the proliferation and migration of metastases cancer cells and induce apoptosis of metastases cancer cells has increased gradually.Conclusion DSF/Cu can inhibit the proliferation and migration of brain metastases from lung adenocarcinoma and induce apoptosis.DSF is expected to be a new method of the combined treatment of brain metastases cancer.