摘要
为脂肪沉积转录调控通路的研究奠定基础,通过生物信息学、Western印迹和RT-qPCR等方法研究肌肉抑制素(MSTN)敲除后对潜在调控脂肪沉积的肌肉增强因子2(MEF2C)和miR-222的表达调控作用。结果表明,在miR-222启动子区域预测到MEF2C的结合位点,Western印迹显示肌肉抑制素敲除后MEF2C表达量上调,qPCR结果表明当MEF2C高表达后miR-222表达量上调3.48倍(P<0.01)。说明MSTN敲除后引起MEF2C高表达,进而促使miR-222表达量上升。
The regulation effect of MSTN knockout on expression of myocyte enhancer factor gene 2(MEF2C)and miR-222 was studied by using bioinformatics,Western blot and RT-qPCR methods to lay the foundation for research of fat deposition transcriptional regulation pathways.Result:The binding site of MEF2C can be predicted in miR-222 promoter region.The Western blot shows that MEF2C expression is up-regulation after MSTN knockout.The qPCR results show that miR-222 expression increases by 3.48 times(P<0.01)after high expression of MEF2C,which indicates that the high expression of MEF2C caused by MSTN knockout can promote rise of miR-222 expression.
作者
肖伟
蔡刚志
华再东
任红艳
朱喆
肖红卫
郑新民
顾玉芳
毕延震
杨玉莹
XIAO Wei;CAI Gangzhi;HUA Zaidong;REN Hongyan;ZHU Zhe;XIAO Hongwei;ZHENG Xinmin;GU Yufang;BI Yanzhen;YANG Yuying(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025;Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064;Key Laboratory of Animal Genetics,Breeding and Reproduction in Plateau Mountainous Region,Ministry of Education,Guiyang,Guizhou 550025;Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction,Guiyang,Guizhou 550025;College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China)
出处
《贵州农业科学》
CAS
2019年第1期83-86,89,共5页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目"猪MSTN通过MEF2C/miR222/SCD5特有分子通路调控脂肪酸代谢的机理"(31772577)
湖北省自然科学基金项目"零风险基因编辑家畜育种技术研究"
"杰出青年"(2018CFA043)
湖北省农业科技创新中心项目"家畜高效繁育技术研究"(2018-620-004-001)
湖北省农业科学院领军人才培养计划项目"猪基因编辑育种技术研究"
