补肾壮骨颗粒对去势雌性大鼠骨组织雌激素受体蛋白表达的影响

Effects of Bushen Zhuanggu granules on expression of estrogen receptor protein in bone tissue of ovariectomized rats

目的探讨补肾壮骨颗粒对去势雌性大鼠骨组织雌激素受体α(ERα)、雌激素受体β(ERβ蛋白表达的影响。方法将60只6月龄SPF级SD雌性大鼠随机均分为模型组(OVX组)、假手术组(SHAM组)、中药组(CM组)、戊酸雌二醇组(EV组)、雷洛昔芬组(RLX组)。各组给药3个月后,每组随机选取半数大鼠测定其脊柱及股骨颈骨密度(BMD),腹主动脉采血处死,立即保留血清并取其左侧股骨,进行血清雌二醇(E2)含量及骨组织ERα及ERβ蛋白表达水平的检测,给药6个月后采用相同方法处理剩余大鼠。结果与SHAM组比较,各阶段用药后,OVX组脊柱及股骨颈BMD值均下降(P <0.05,P <0.001)。与OVX组相比,用药3个月后CM组脊柱及股骨颈BMD值均提高,差异有统计学意义(P <0.01,P <0.05);6个月后各用药组脊柱BMD值均升高(P <0.05),股骨颈BMD值亦提高,其中CM组提高较明显,差异有统计学意义(P <0.05)。与SHAM组相比,两阶段给药后OVX组ERα、ERβ水平均显著降低(P <0.001),与OVX组比较,各用药组两种蛋白水平均显著提高(P <0.001)。用药3个月后,3个用药组之间比较差异无统计学意义(P> 0.05);6个月后,CM组ERα蛋白水平高于RLX组(P <0.05),与EV组比较差异无统计学意义(P> 0.05)。给药3、6个月后,CM组E2含量较OVX组均有提高(P <0.05)。结论补肾壮骨颗粒对去卵巢雌鼠骨组织可能具有类雌激素样作用,且可在一定程度上提高ERα、ERβ蛋白表达水平,提高去卵巢大鼠BMD。

Objective To investigate the effects of Bushen Zhuanggu granules on the expression of estro gen receptor alpha(ERα)and estrogen receptorβ(ERβ)protein in bone tissue of ovariectomized rats.Meth ods Sixty female SPF-class SD female rats aged six months were randomly divided into ovarian group(OVX),sham operation group(SHAM),Chinese medicine group(CM),estradiol valerate group(EV),and raloxifene group(RLX).After 3 months of administration in each group,half of the rats in each group were randomly select ed to measure the bone mineral density(BMD)of spine and femur.Then the rat abdominal aorta was sacrificed with the serum removed and the left femur was taken immediately for the detection of serum estradiol(E2)content and the expression of ERαand ERβprotein in each group.The remaining rats were treated in the same manner af ter 6 months--administration.Results Compared with those in the SHAM group,the BMD of the spine and femur in the OVX group decreased(P<0.05,P<0.001)after each stage of medication.Compared with those in the OVX group,the BMD of the spine and femoral neck of the CM group were both increased(P<0.01,P<0.05)af ter 3 months.The spinal BMD of each group was increased(P<0.05),meanwhile that of the CM group was much higher than that of the other two groups and the difference was statistically significant(P<0.05).Compared with those in the SHAM group,the levels of ERαand ERβin the OVX group were significantly lower after two-stage of administration(P<0.001).Compared with those in the OVX group,the levels of the two proteins in all drug groups were significantly increased(P<0.001).After 3 months of treatment,there was no significant difference among the three drug groups(P >0.05).And 3 months later,the level of ERαprotein in CM group was higher than that in RLX group(P<0.05),but there was no difference when compared with EV group(P >0.05).The serum E2 content in the CM group was higher than that in the OVX group(P<0.05)after 3 and 6 months.Conclu sions Bushen Zhuanggu Granules may have an estrogen-like effect on the bone tissue of ovariectomized rats,and can increase the expression levels of ERαand ERβproteins to a certain extent and increase the BMD of ovariecto mized rats.