丝氨酸蛋白酶抑制剂B1在肝肺综合征大鼠肺组织中的表达变化

Expression of serine protease inhibitor B1 in lung of rats with hepatopulmonary syndrome

目的探讨丝氨酸蛋白酶抑制剂B1(SerpinB1)在肝肺综合征(HPS)肺组织中的表达情况及其可能存在的调控机制。方法定量PCR免疫组化染色观察SerpinB1、Erk1/2、p-Erk1/2以及微血管内皮细胞表面标志物CD34在肺组织中的分布以及表达。结果 CBDL(CBDL)大鼠PaO_2下调并且A-aDO2升高(P <0.01);HE染色表明CBDL组大鼠肺内有大鼠肺组织SerpinB1、Erk1/2对应的基因及蛋白表达水平随周期升高(P <0.01);免疫组化结果提示SerpinB1主要高表达于CBDL大鼠肺微血管内皮细胞及炎症细胞,CD34阳性细胞在CBDL组表达上调并且与SerpinB1上调呈正相关(r=0.795,P <0.05)。结论 SerpinB1大鼠肺组织中的表达显著上调,与激活Erk1/2并与肝肺综合征肺血管新生的形成有关。

Objective To investigate the expression of serpin protease inhibitor B1(SerpinB1)in lung of hepatopulmonary syndrome(HPS)rats and the potential mechanism underlying serpin B1 regulation.Methods The mRNA and protein expression of SerpinB1 in lung was detected by RT-PCR and Western blot assay.The distribution and expression of SerpinB1,Erk1/2,p-Erk1/2 and CD34(the marker of microvascular endothelial cell)in lung tissue were observed by immunohistochemistry.Immunofluorescence was used to detect the proliferation of pulmonary microvasculature and the expression of SerpinB1.Results PaO2 decreased and A-aDO2 increased significantly in CBDL rats(common bile duct ligation,CBDL)(P﹤0.01).Results of HE staining showed the infiltration of inflammatory cells and thickening of the alveolar septum in lung in CBDL group.The results of qPCR and western blot showed that SerpinB1 expression in lungs of CBDL rats increased in a time-dependent manner(P﹤0.01).The results of immunohistochemical staining revealed that serpinB1 was mainly expressed in lung microvascular endothelial cells and inflammatory cells in CBDL rats.Conclusions The expression of SerpinB1 in lung tissue of HPS rats was significantly up-regulated,which may be related to the activation of Erk1/2 and the formation of pulmonary angiogenesis in HPS rats.