高表达SEMG1蛋白的弱精子症大鼠模型的建立

Establishment of rat model of asthenozoospermia with high expression of SEMG1 protein

目的构建一个睾丸中SEMG1蛋白高表达的弱精子症大鼠模型。方法将30只SD大鼠随机分为3组:空白对照组、阴性对照组、及实验组(腹腔注射环磷酰胺),每组10只。实验结束后称其体质量及一侧睾丸质量并计算睾丸指数;睾丸常规石蜡切片,HE染色;取附睾尾制备精子悬液进行精子计数及活力检测; Western blot检测SEMG1及CASPASE-3及BCL-2蛋白表达。结果与对照组相比,实验组大鼠体质量差异无统计学意义(P> 0.05),睾丸质量及睾丸指数较对照组降低(P <0.001),实验组大鼠精子悬液镜下观察见精子数量及精子活力明显降低,HE染色见睾丸生精小管中各级生精细胞排列紊乱,细胞数量减少。Western blot结果显示:与对照组相比,实验组SEMG1及CASPASE-3蛋白表达水平显著升高,BCL-2蛋白表达水平明显降低(P <0.001)。结论成功构建了睾丸中高表达SEMG1蛋白的弱精子症大鼠模型。

Objective To construct a rat model of asthenozoospermia with high expression of SEMG1 gene in testis.Methods Thirty SD rats were randomly divided into 3 groups,10 in each group.The blank control group which did not do any treatment,the saline intraperitoneal injection group and the cyclophosphamide intraperitoneal injection group.At the end of the experiment,the quality and the testicular index was calculated.The testis was routinely paraffin-embedded and HE staining.The spermatic suspension was prepared for sperm count.The expression of SEMG1,CASPE-3 and BCL-2 protein was detected by Western blot.Results Compared with the control group,there was no significant difference in the quality of the cyclophosphamide-treated group(P﹥0.05).The testicular mass and testicular index were lower than the control group(P﹤0.001).Under the microscope,the number of spermatozoa was significantly reduced in the cyclophosphamide-treated group.In HE,the spermatogenic cells in the spermatogenic tubules were disordered and the number of cells was reduced.Western blot results showed that compared with the control group,the expression levels of SEMG1 and CASPE-3 in the cyclophospha-mide treatment group were significantly increased,and the expression level of BCL-2 protein was significantly decreased(P﹤0.001).Conclusion A rat model of asthenzoospermia with high expression of SEMG1 gene in testis is successfully constructed.