花期高温胁迫下TaERECTA基因对小麦生理特性和光合作用的影响

Effect of TaERECTA on Physiological Traits and Photosynthesis in Wheat under High-Temperature Stress at Anthesis

为研究花期高温胁迫对转TaERECTA基因小麦生理特性和光合作用的影响,在扬花期对转TaERECTA基因小麦进行高温胁迫,测定转基因小麦的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性以及丙二醛(MDA)含量和相对电导率,并于扬花期测定其光合相关参数。结果显示,随着高温胁迫时间的延长,转基因小麦叶片的POD和CAT活性呈明显下降趋势,而SOD活性较稳定,MDA含量和相对电导率增加;与对照株系相比,高温胁迫处理后,转基因小麦株系具有较高的抗氧化酶活性,较低的MDA含量和相对电导率;高温胁迫显著降低了小麦植株的SPAD值,且转基因小麦的光合速率和瞬时水分利用效率均显著大于对照株系。说明TaERECTA基因可以参与调控小麦叶片组织细胞膜的稳定性,改良高温胁迫下小麦的光合能力和水分利用效率。

In order to investigate the influence of TaERECTA on physiological traits and photosynthesis in wheat under high-temperature stress at anthesis,the activity of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),content of MDA and relative electronic conductivity in TaERECTA overexpressing plants were investigated.The photosynthetic parameters at flowering stage were determined.Under high-temperature stress,the results showed that the activity of POD and CAT in TaERECTA overexpressing plants showed a dramatically decrease with the extension of treatment,whereas the activity of SOD showed stably,and content of MDA and relative electronic conductivity were gradually increased.Comparing to control,TaERECTA overexpressing plants showed higher antioxidase activity,lower content of MDA and relative electronic conductivity;SPAD was significantly reduced due to high-temperature stress,and photosynthesis rate and instant water use efficiency(WUEi)of TaERECTA overexpressing plants were significantly higher than those of control plants.It is suggested that TaERECTA was involved in regulating the cell membrane stability of wheat leaves,which can improve their photosynthetic capacity and water use efficiency under high-temperature stress.