(6,5)手性单壁碳纳米管的双水相分离及其对紫杉醇的载药研究

Separation of (6,5) Chiral Single-Wall Carbon Nanotube by an Aqueous Two-Phase System and Its Application on Paclitaxel Loading

采用聚乙二醇/葡聚糖组成的双水相系统(ATP),结合盐酸辅助调节分配的方法,实现了单一(6,5)手性单壁碳纳米管((6,5)SWCNT)的分离。将分离所得的(6,5)SWCNT分散于牛血清白蛋白(BSA)中,并添加紫杉醇(PTX)制备得到(6,5)SWCNT-BSA-PTX三元复合物。采用HeLa细胞对三元复合物进行细胞毒性评价,结果表明,(6,5)SWCNT作为药物载体能够显著降低PTX的半抑制剂浓度(IC_(50 ))至7.1×10^(-9) mol/L。

A method for separation of(6,5)chiral single-wall carbon nanotube(SWCNT)was reported.The separation was achieved by an aqueous two-phase system(ATP)consisting of polyethylene glycol/dextran in the presence of hydrochloric acid(HCl)and surfactants.Paclitaxel(PTX)has been used in the clinical treatment of breast cancer,ovarian cancer,and etc.,which has poor water solubility and low toxic side effects.The non-covalent adsorption of PTX with bovine serum albumin(BSA)resolves the issue of poor water solubility for PTX.The PTX adsorbed BSA can also be coated on the surface of SWCNTs to become a(6,5)SWCNT-BSA-PTX ternary complex through non-covalent modification of SWCNTs,which leads to the enhancement of cell penetration.The cytotoxicity of the ternary complex was evaluated and the results showed that(6,5)SWCNT as a promising drug carrier could significantly reduce the IC50 of PTX to 7.1× 10^-9 mol/L.It can be concluded that the method established in this work does not require complex covalent functionalization of SWCNTs and biocompatibility of the materials is significantly improved by involving BSA.The toxic effects of PTX is greatly improved for HeLa cells by separated single chiral(6,5)SWCNTs,which should have applications for delivery of other drugs.