家蝇胚胎细胞抗菌肽对人髓样白血病细胞K562的杀伤作用

Inhibition of antibacterial peptide from embryonic cells of Musca domestica on human myeloid leukemia cell K562

目的探讨家蝇胚胎细胞抗菌肽和高三尖杉酯碱(HHT)对人髓样白血病细胞K562的抑制、杀伤作用。方法采用脂多糖诱导家蝇胚胎细胞,提取抗菌肽。采用磷酸盐缓冲液(PBS)配制80、160、320及640μg/mL4个浓度,阴性对照组仅加入PBS和培养基。采用流式细胞术检测不同浓度抗菌肽对K562细胞周期和细胞凋亡率的影响。用处于对数生长阶段的K562和人脐静脉血管内皮细胞制备成浓度相同的2个药物组(HHT组和抗菌肽组),不加入药物的2种细胞作为正常对照组。采用流式细胞术检测正常对照组及2个药物组对2种细胞的有效杀伤率。结果 4个浓度组抗菌肽均使K562的S期降低,增殖指数降低,并引起G0/G1期升高,与阴性对照组相比差异有统计学意义(P<0.05)。4个浓度组K562细胞凋亡率与阴性对照组相比差异无统计学意义(P>0.05)。HHT组和抗菌肽组对K562的有效杀伤率显著升高,HHT组和抗菌肽组与正常对照组比较差异有统计学意义(P<0.05)。HHT组对人脐静脉血管内皮细胞的有效杀伤率显著高于正常对照组和抗菌肽组(P<0.05)。结论家蝇胚胎细胞抗菌肽能有效抑制肿瘤细胞的生长,对正常人体细胞无抑制、杀伤作用。

Objective To study the inhibition of antibacterial peptide from embryonic cells of Musca domestica and homoharringtonine(HHT)on human myeloid leukemia cell K562.Methods The embryonic cells of Musca domestica were induced using lipopolysaccharide.Antibacterial peptide was extracted and prepared 80,160,320 and 640 μg/mL level groups by phosphate-buffered saline(PBS).The medium only adding PBS was used as negative control group.The cell cycle and the cell apoptosis ratio of K562 were determined by flow cytometry.The K562 and human umbilical vein vascular endothelial cells during logarithmic growth phase were prepared as HTT and antibacterial peptide groups.K562 and human umbilical vein vascular endothelial cells without treatment were used as normal control group.The effective inhibition ratios of HHT and antibacterial peptide on K562 and human umbilical vein vascular endothelial cells were determined by flow cytometry.Results Compared with negative control group,the G0/G1 of K562 was increased,the S was decreased,and the proliferation index was decreased(P<0.05).Compared with negative control group,the cell apoptosis ratio of K562 in various level groups had no statistical significance(P >0.05).The effective inhibition ratios of HHT group and antibacterial peptide group on K562 were increased and had statistical significance compared with normal control group(P<0.05).The effective inhibition ratio in HHT group on human umbilical vein vascular endothelial cells was higher than those in normal control and antibacterial peptide groups(P<0.05).Conclusions Antibacterial peptide from embryonic cells of Musca domestica can inhibit tumor cells effectively,but no damage to normal cells.