烟草中甲霜灵残留快速检测试纸条研制

Development of A Rapid Test Strip for Determination of Metalaxyl Residues in Tobacco

为开发快速检测烟草(Nicotiana tabacum L.)中的甲霜灵残留方法,以自制抗原、抗体为材料,研制一种胶体金免疫层析检测试纸条,并以甲霜灵原料合成半抗原,进一步制备抗原免疫后制备甲霜灵单克隆抗体。结果表明,纸条检测限对干烟草为1.5 mg/kg,鲜烟叶为1.0 mg/kg,与其他药物无交叉反应,且与色谱法比较符合率为98%,假阳性率为2.3%。甲霜灵残留试纸条检测烟草时样品处理简单、检测时间短、无需专业人员、经济适用等特点,能够实现烟草快速、准确、批量检测,可为烟草中甲霜灵残留检测提供技术支持。

In order to develop a method for rapid detection of metalaxyl residues in tobacco,a colloidal gold immunochromatography test strip was developea by using the self-made antigen and antibody as the material.The hapten was synthesized from metalaxyl and further prepared monoclonal antibody against metalaxyl after antigen immunization.The test strip limits the test limit to dry tobacco 1.5 mg/kg,fresh tobacco leaves 1.0 mg/kg,test paper stable,no cross reaction with other drugs,and the comparison with the chromatographic method is 98%,false positive rate is 2.3%.It can be used to detect tobacco quickly,accurately and batch,and provide technical support for the detection of methfrol residue in tobacco.