变换波长HPLC法同时测定药食同源降脂片中橙黄决明素、芦丁和槲皮素含量

Wavelength Conversion HPLC Determination of Aurantio-obtusin,Rutin and Quercetin in Lipid-lowering Chinese Medicines

采用Gemini C_(18)色谱柱(4.6 mm×250 mm,5μm),流速为1 mL/min,柱温40℃,波长切换(0～20 min,在370 nm波长下检测芦丁,20～30 min,在280 nm波长下检测槲皮素和橙黄决明素),进样量20μL,流动相为甲醇(A)-水(B)。结果表明,橙黄决明素在1.977 2～98.860 0μg/mL(R^2=0.999 7)线性关系良好,平均回收率为98.81%,RSD为2.0%;芦丁在1.9580～97.9000μg/mL(R^2=0.999 6)线性关系良好,平均回收率为98.16%,RSD为2.2%;槲皮素在2.283 6～114.18μg/mL(R^2=0.999 6)线性关系良好,平均回收率为100.44%,RSD为3.0%。药食同源降脂片中橙黄决明素平均含量0.053 2 mg/g,RSD为1.36%;芦丁平均含量0.847 0 mg/g,RSD为0.65%;槲皮素平均含量0.040 9 mg/g,RSD为0.80%。该方法操作简便、准确、稳定、重复性好,可用于药食同源降脂片的质量控制。

Aurantio-obtusin,rutin and quercetin were separated on Gemini C18 RP-column(4.6 mm×250 mm,5μm),the flow rate was 1.0 mL/min,column temperature was 40℃.The detection wavelength was set at 370 nm for rutin’s detection in the first 20 minutes,and then changed into 280 nm for the other two ingredients’s detection in 20~30 minutes.The injection volume was 20μL,with methyl alcohol as the mobile phase A and water as mobile phase B.Results showed that aurantio-obtusin,rutin and quercetin showed good linear relationships in the range of 1.977 2 to 98.860 0μg/mL(R^2=0.999 7),1.958 0 to 97.900 0μg/mL(R^2=0.999 6)and 2.283 6 to 114.18μg/mL(R^2=0.999 6).Their average recovery rates were 98.81%with RSD of 2.0%,98.16%with RSD of 2.2%and 100.44%with RSD of 3.0%.By measurement,the content of aurantio-obtusin,rutin and quercetin in lipid-lowering Chinese medicines were 0.053 2 mg/g with RSD of 1.36%,0.847 0 mg/g with RSD of 0.65%,and 0.040 9 mg/g with RSD of 0.80%.The method is simple,precise,stable,reproducible,and it can be used for the quality control of lipid-lowering Chinese medicines.