慢性肾脏病湿热型体质易感人群ICAM1基因和IL-10基因启动子多态性研究

Study on ICAM1 Gene and IL-10 Gene Promoter Polymorphism of Damp-heat Constitution People Susceptible to Chronic Kidney Disease

目的:探讨岭南地区湿热型体质慢性肾脏病(CKD)1期患者ICAM1基因和白细胞介素10(IL-10)基因启动子的多态性。方法:选择106例CKD1期患者,随机分为对CKD1期湿热质组(50例)、CKD1期平和质组(56例);选择102例健康体检者,按体质分为健康湿热质组(41例)、健康平和质组(61例)。利用荧光定量PCR方法和ELISA检测方法进行IL-10基因启动子-1082、-819、-592位点多态性研究及ICAM1基因G241和K469位点的多态性分析,并且检测ICAM1、IL-10血清浓度及对比分析。结果:4组IL-10基因启动子、ICAM1基因各位点基因型分布及基因频率比较,差异无统计学意义(P>0.05),4组IL-10血清浓度比较差异无统计学意义(P>0.05),4组ICAM1血清浓度比较差异有统计学意义(P<0.05),且含有G241位点GG基因型血清ICAM1浓度显著比含有GR基因型低,差异有统计学意义(P<0.05),含有K469位点不同基因型血清ICAM1浓度比较差异无统计学意义(P>0.05)。结论:湿热体质人群对CKD具有易感性,ICAM-1 G241位点GG基因型可能是CKD湿热体质预测基因,且通过G241位点GG基因型调控ICAM1,使得ICAM1的表达下调,血清ICAM1浓度降低,减少炎症反应。

Objective:To discuss the promoter polymorphism of ICAM1 gene and IL-10 gene of damp-heat constitution patients suffering chronic kidney disease(CKD)at stage one in Lingnan district.Methods:All 106 CKD1 patients were chosen and randomized into the group of damp-heat constitution CKD1(50 cases)and the group of peaceful constitution CKD1(56 cases);another 102 people who accepted physical examinations were selected and randomized into health group of damp-heat constitution(41 cases)and health group of peaceful constitution(61 cases).Polymorphism of ICAM1 gene G241 and K469 loci were analyzed and polymorphism of IL-10 gene promoters-1082,-819 and-592 loci were studied by fluorescent quantitation PCR methods and ELISA method,the concentrations of ICAM1 and IL-10 were detected,compared and analyzed.Results:The difference had no statistical meaning in the comparisons of genotype distribution and gene frequencies of IL-10 gene promoter and ICAM1 gene(P >0.05),the difference had no statistical meaning in the comparisons of the concentrations of IL-10 among four groups(P >0.05),the difference was statistically significant in the comparisons of the concentrations of ICAM1 among four groups(P<0.05),and the concentrations of ICAM1 in GG genotype serum containing G241 loci were lower than these in GR genotype serum,and the difference had statistical meaning(P<0.05),the difference had no statistical meaning in the comparisons of the concentrations of ICAM1 in different genotypes serums containing K469 loci(P >0.05).Conclusion:The people in damp-heat constitution have the susceptibility to CKD,ICAM1-1 G241 loci GG genotype might be the gene predicting damp-heat constitution CKD,and it could regulate ICAM1 by GG genotype G241 loci,reduce the expressions of ICAM1,decrease the concentrations of ICAM1 and relieve inflammatory reaction.