大麦黄花叶病抗性的遗传分析与QTL定位

Genetic Analysis and QTL Mapping of Resistance to Yellow Mosaic Disease in Barley(Hordeum vulgare L.)

大麦黄花叶病是依靠土壤中禾谷多黏菌传播的病毒病,严重影响冬大麦的产量和品质,培育和利用抗病品种是最经济有效的防治方法。本研究以抗病品种扬饲麦1号与感病品种Gairdner为亲本,以杂交F1代经花药离体培养技术构建的DH群体为材料,对其大麦黄花叶病抗性进行两年鉴定与分析,并利用91对在亲本间多态性好的SSR(simple sequence repeat)标记构建了群体的遗传连锁图谱,采用Windows QTL IciMapping 4.0软件中的完备区间-加性模型(ICIM-ADD)对大麦黄花叶病进行QTL定位,共检测到9个与大麦黄花叶病病情指数相关的QTLs,其中,qRYM-1Ha、qRYM-1Hc、qRYM-2Ha及qRYM-2Hb在两年间均被检测到,且qRYM-2Ha在两年6个时期均被检测到,位于EBmag0793至GBM1047标记区间内,可解释的表型变异为5.61%~38.04%;qRYM-2Hb在2015年第二、三期和2016年第一期被检测到,位于GBM1047至Bmag0749标记区间内,可解释的表型变异为11.40%~18.80%。qRYM-1Ha和qRYM-4Ha可能是两个新的大麦黄花叶病抗性QTLs,黄花叶病抗性基因均来源于黄花叶病抗性品种扬饲麦1号。本研究为大麦黄花叶病抗性基因的发掘、精细定位、基因克隆及分子标记辅助选择育种提供了有利信息。

Barley yellow mosaic disease is a kind of virus disease which relies on the transmission of Polymyxa graminis in the soil,seriously decreasing the yield and quality of winter barley.Resistant cultivars are the most cost effective measure for controlling the disease.In this paper,a total of 173 doubled-haploid(DH)lines that generated from a cross between a disease-resistant Chinese landrace barley Yangsimai 1 and a susceptible cultivar barley Gairdner were used.The resistance to barley yellow mosaic disease was identified and analyzed by using DH population and its parents across two years.A genetic linkage map including 91 pairs of SSR markers with obvious polymorphism between the parents was constructed.QTL mapping for barley yellow mosaic disease was carried out by using the complete interval-additive model(Inclusive Composite Interval-Additive Mapping,ICIM-ADD)method in Windows QTL IciMapping 4.0 software.The result showed that nine QTLs for the disease index of barley yellow mosaic disease were identified,of which four QTLs(qRYM-1 Ha,qRYM-1 Hc,qRYM-2 Haand qRYM-2 Hb)were detected across two years.qRYM-2 Hawas detected in six stages across two years,and located in the EBmag0793-GBM1047 intervals,which explained 5.61%-38.04% of barley yellow mosaic disease resistant variation;qRYM-2 Hb was detected in the second and third stages of 2015 and the first stage of 2016,and located in the GBM1047-Bmag0749 intervals,which explained 11.40%-18.80% of barley yellow mosaic disease resistant variation.Two putative QTLs,qRYM-1 Ha and qRYM-4 Halikely represented new loci for barley yellow mosaic disease resistance.Notably,the resistance genes of barley yellow mosaic disease were all derived from the resistant variety Yangsimai 1.The results have supplied valuable information for fine mapping,cloning genes and further molecular-marker-assisted selection breeding of barley yellow mosaic disease.