XP-endo Finisher锉对根管内壁玷污层清除效果的体外研究

In vitro evaluation of the effectiveness of XP-endo Finisher file on smear layer removal after root canal instrumentation

目的评估XP-endo Finisher(XPF)锉与被动超声冲洗(PUI)对根管内壁玷污层的清除效果。方法选择60颗离体单直根管下颌前磨牙,距根尖16 mm处截冠,采用S3镍钛锉预备到3S,根据终末处理方式不同将样本随机分成6组。A组:3 mL 3%次氯酸钠溶液联合XPF处理1 min;B组:3 mL 3%次氯酸钠溶液联合XPF处理1 min,再用4 mL 17%乙二胺四乙酸(EDTA)溶液冲洗1 min;C组:3 mL 3%次氯酸钠溶液联合PUI处理1 min;D组:3 mL 3%次氯酸钠溶液联合PUI处理1 min,再用4 mL 17%EDTA溶液冲洗1 min;E组:3 mL 3%次氯酸钠溶液30号侧方冲洗针冲洗1 min;F组:3 mL 3%次氯酸钠溶液30号侧方冲洗针冲洗1 min,再用4 mL 17%EDTA溶液冲洗1 min。采用扫描电子显微镜观察玷污层形态,并比较根尖1/3区、根中1/3区牙本质小管开口数。结果 A、C、E组实验样本整个根管壁都有玷污层覆盖,A组与C组牙本质小管开口数明显高于E组(P<0.05),而A组与C组的差异无统计学意义(P>0.05);F组根中区、B组和D组根尖区有少量玷污层覆盖,牙本质小管开放或半开放;F组根尖区可见玷污层,牙本质小管封闭或半封闭;B和D组根中区根管壁玷污层均被有效去除;在根尖1/3区和根中1/3区,B组与D组牙本质小管开口数高于其他4组(P<0.05),而B组和D组间的差异无统计学意义(P>0.05)。结论 XPF锉对根管内壁玷污层的清洁效果与PUI无明显差异,可用于根管预备后提高根管清洁效果。

Objective This study aimed to evaluate the effectiveness of XP-endo Finisher(XPF)file and passive ultrasonic irrigation(PUI)in the smear layer removal of the root canal.Methods A total of 60 human single-rooted premolars were selected and decoronated to standardize their canal length to 16 mm.Tooth samples were prepared using a S3 rotary system to prepare root canal with the file size of 3S and then randomly divided into 6 groups according to the final irrigation protocol,as follows:XPF 3 mL of 3%NaOCl for 1 min(group A);XPF 3 mL of 3%NaOCl for 1 min,followed by 4 mL of 17%ethylene diamine tetraacetic acid(EDTA)for 1 min(group B);PUI of 3 mL of 3%NaOCl for 1 min(group C);PUI of 3 mL of 3%NaOCl for 1 min,followed by 4 mL of 17%EDTA for 1 min(group D);3 mL of 3%NaOCl for 1 min by using a syringe and a 30 G side-vented needle(group E);and 3 mL of 3%NaOCl for 1 min by using a syringe and a 30 G side-vented needle,followed by 4 mL of 17%EDTA for 1 min(group F).After the completion of the root canal preparation,the teeth were split into two longitudinally.The mean numbers of the visible open dentinal tubules in the apical and middle thirds of the root canals were evaluated via scanning electron microscope.Results The whole surfaces of the root canals in groups A,C,and E were covered by a smear layer.Groups A and C possessed significantly higher number of visible open dentinal tubules than in group E(P<0.05),with statistically insignificant difference between groups A and C(P >0.05).The apical third of the samples in groups B and D and in the middle thirds of canals in group F exhibited a small amount of smear layer,and the dentinal tubules were open or semi-open.The root canal surfaces in the apical third of the samples in group F were covered by a smear layer,and the dentinal tubules were sealed or semisealed.The smear layers in the middle third of the samples in groups B and D were removed,and the dentinal tubules were more visibly open than those of the four other groups(P<0.05).The difference between groups B and D were statistically insignificant(P >0.05).Conclusion The difference between XPF and PUI in terms of the smear layer removal of the root canals was insignificant.Hence,XPF,as a new irrigation agitation technique,can aid in improving smear layer removal.