农村有机生活垃圾沼气发酵工艺优化及菌群分析

Optimization of Biogas Fermentation Process and Microflora Analysis of Rural Organic Household Waste

为了了解沼气发酵对农村有机生活垃圾中细菌菌群的影响,文章从2个正在稳定运行的20 L反应器中选取两个水力停留时间(15 d,20 d)进行实验。实验结果表明,农村有机生活垃圾在2个水力停留时间内,沼气产量随水力停留时间的缩短而提高;沼气中甲烷成分均大于50%,粒径大的反应器中沼气甲烷成分要高于粒径小的反应器;在所选4批次的原料中,存在的病原菌为Enterobacter和Enterococcus,未发现Salmonella,Shigella Castellani及Staphylococcus Aureus,随着沼气发酵的进行,原料中腐败性细菌减少,发酵性细菌增加。

In order to understand the effect of biogas fermentation on bacterial flora in the rural organic waste,two 20 L reactors in stable operation were selected and two hydraulic retention times(15 d,20 d)were experimented.The experimental results showed that,from the tested 2 hydraulic retention time,the biogas production increased with the shortening of the hydraulic retention time.The methane content in biogas was greater than 50%,and the methane content in the reactor with larger particle size was higher than that with smaller particle size.In the 4 batches of raw materials selected,there existed pathogenic bacteria Enterobacter and Enterococcus,no Salmonella,Shigella Castellani and Staphylococcus Aureus were found.As the biogas fermentation went on,pathogenic bacteria became less,and fermentation bacteria became more.