摘要
目的研究乙型肝炎病毒X蛋白(HBx)对肝癌细胞增殖的影响,并探索HBx过表达对肝癌细胞PI3K/AKT表达的影响。方法构建过表达HBx的肝癌HepG2细胞,测定其荧光素酶的表达,采用MTT法研究肝癌细胞增殖情况,采用Western blotting法对各组细胞PI3K及AKT的表达情况进行研究。结果成功构建了过表达HBx的肝癌HepG2细胞,HBx有促进肝癌细胞增殖的作用,进一步研究发现,过表达HBx有上调肝癌细胞PI3K及AKT表达的作用。结论 HBx有促进肝癌细胞增殖作用,其机制可能与上调PI3K/AKT表达有关。
Objective To study the effect of HBx on the proliferation of hepatocellular carcinoma cells, and to explore its relationship with PI3K/AKT. Methods Overexpression of HBx in HepG2 cells was constructed and their luciferase expression was determined. MTT method was used to study the proliferation of hepatocellular carcinoma cells. Western blotting was used to study the expressions of PI3K and AKT in each group. Results The overexpression of HBx in HepG2 cells was constructed successfully, which promoted the proliferation of hepatocellular carcinoma cells. Further studies showed that overexpression of HBx could up-regulate the expressions of PI3K and AKT. Conclusion HBx has the effect of promoting the proliferation of hepatocellular carcinoma cells, and its mechanism may be related to the up-regulation of PI3K/AKT expression.
作者
张斌
丁慎华
徐春江
许智玲
朱薇珊
ZHANG Bin;DING Shenhua;XU Chunjiang;XU Zhiling;ZHU Weishan(Department of Liver Disease,Fudan University ,Shanghai 201700,China;Department of Hyperbaric Oxygen,Qingpu Branch of Zhongshan Hospital,Fudan University ,Shanghai 201700,China)
出处
《胃肠病学和肝病学杂志》
CAS
2019年第2期159-162,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
上海市卫生计生委基金资助项目(市卫计委201540130)
上海市青浦区科委基金资助项目(青科发2015-12)
