IL-12RB2 rs3790567A/G位点单核苷酸多态性与原发性胆汁性肝硬化易感性的Meta分析

Association between the IL-12RB2 rs3790567A/G polymorphism and the susceptibility of primary biliary cirrhosis: a Meta-analysis

目的评价IL-12RB2 rs3790567A/G位点单核苷酸多态性与原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)易感性的关系。方法通过计算机检索Pub Med、Embase、the Cochrane Library、Web of Science、中国知网、万方等中英文数据库,检索IL-12RB2 rs3790567A/G位点单核苷酸多态性与PBC相关的病例-对照研究。以PBC组与对照组人群基因型频率的OR值和95%CI为效应指标,采用随机或固定效应模型进行定量合并分析,并进行偏倚评估和敏感性分析。采用Rev Man 5. 3软件进行Meta分析。结果本研究共纳入文献6篇,PBC组(病例组) 1 584例,健康对照组2 648名。Meta分析结果表明,IL-12RB2 rs3790567A/G位点与PBC发病因素密切相关。5个基因型结果如下:显性模型:AA+AG vs GG,OR=1. 20,95%CI:1. 11～1. 29,差异有统计学意义(P <0. 05);隐性模型:AA vs AG+GG,OR=1. 59,95%CI:1. 25～2. 02,差异有统计学意义(P <0. 05);共显性模型:AG vs AA,OR=0. 83,95%CI:0. 53～1. 32,差异有统计学意义(P <0. 05); GG vs AA,OR=0. 03,95%CI:0～0. 07,差异有统计学意义(P <0. 05);等位基因模型A vs G,OR=1. 02,95%CI:0. 72～1. 45,差异无统计学意义(P> 0. 05)。以研究地理位置及种族不同进行亚组分析,亚洲人显性基因模型:AA+AG vs GG,OR=1. 17,95%CI:0. 89～1. 55,差异无统计学意义(P=0. 26)。高加索人显性基因模型:AA+AG vs GG,OR=1. 30,95%CI:1. 17～1. 44,差异有统计学意义(P <0. 05);亚洲人隐性基因模型AA vs AG+GG,OR=1. 39,95%CI:1. 01～1. 91,差异无统计学意义(P=0. 05)。高加索人隐性基因模型:AA vs AG+GG,OR=1. 94,95%CI:1. 34～2. 81,差异有统计学意义(P <0. 05);亚洲人等位基因模型A vs G:OR=1. 26,95%CI:0. 70～2. 26,P> 0. 05,高加索人A vs G,OR=1. 02,95%CI:0. 72～1. 45,差异无统计学意义(P> 0. 05)。结论 IL-12RB2 rs3790567A/G在PBC AA和AA+AG基因模型中易感性显著增加,且通过亚组分析,证明种族间差异有统计学意义。

Objective To determine the association between IL-12 RB2 rs3790567A/G polymorphism and the susceptibility of primary biliary cirrhosis (PBC). Methods The literatures in the PubMed, the Cochrane Library, Embase , Web of Science, CNKI, WanFang databases were searched. Quality assessment of the included studies was performed , and relevant information was collected. The odds ratios ( OR ) and 95% confidence intervals (95% CI ) were assessed by the Review Manager version 5.3 software, subgroup and sensitivity analysis were conducted to explore potential sources of heterogeneity. Results There were 6 studies that included 1 584 cases and 2 648 controls. There were significant associations between IL-12 RB2 rs3790567 A/G polymorphism and PBC risk factors. Dominant model: AA+AG vs GG, OR =1.20, 95% CI : 1.11-1.29, the difference was statistically significant ( P <0.05);recessive model: AA vs AG+GG, OR= 1.59 , 95% CI : 1.25-2.02, the difference was statistically significant ( P <0.05);codominant model: AG vs AA, OR=0.83, 95% CI : 0.53-1.32, there was significantly difference ( P <0.05);GG vs AA, OR=0.03, 95% CI: 0-0.07, there was significant difference ( P <0.05);allelic model: A vs G, OR= 1.02 , 95% CI: 0.72-1.45, the difference was not statistically significant ( P >0.05). The different results were obtained in the subgroup analysis by ethnicity, Asian dominant model: AA+AG vs GG, OR=1.17, 95% CI: 0.89 - 1.55 , P = 0.26, and recessive model: AA vs AG+GG, OR=1.39, 95% CI: 1.01-1.91, P =0.05;allelic model: A vs G, OR=1.26, 95% CI : 0.70-2.26,P >0.05. Dominant gene model of Caucasians: AA+AG vs GG, OR= 1.30 , 95% CI: 1.17-1.44, P <0.05;recessive gene model of Caucasians: AA vs AG+GG, OR= 1.94 , 95% CI : 1.34 -2.81, the difference was statistically significant ( P <0.05);allelic model: A vs G, OR= 1.02 , 95% CI: 0.72- 1.45 , the difference was not statistically significant ( P >0.05). Conclusion IL-12RB2 rs3790567 A/G in PBC AA and AA+AG gene model increased susceptibility significantly, and by subgroup analysis, there was no racial difference in Caucasians and Asians.