术后24 h给予莫诺苷对局灶性脑缺血再灌注大鼠保护作用的研究

Protective effects of morroniside in rats at 24 h after focal cerebral ischemia-reperfusion

目的研究莫诺苷对大鼠局灶性脑缺血再灌注后24 h给药对血管新生相关因子及神经功能的影响。方法健康成年雄性SD大鼠,用线栓法制备大鼠局灶性脑缺血再灌注模型,术后随机分为假手术组,模型组,莫诺苷小(30 mg/kg)、中(90 mg/kg)、大(270 mg/kg)剂量组,术后24 h开始给予莫诺苷,每天灌胃1次,连续给药7 d。通过检测神经功能行为学评分,脑梗死体积百分比,皮层血管新生相关蛋白的表达,研究大鼠局灶性脑缺血再灌注后24 h给予莫诺苷对血管新生相关蛋白及神经功能恢复的影响。结果与假手术组相比,模型组神经功能评分和脑梗死体积百分比显著增加(P<0. 001),给药7 d后,莫诺苷大剂量组与模型组相比,明显降低了神经功能评分(P<0. 01),且能显著降低脑梗死体积(P<0. 05)。与假手术相比,皮层血管新生相关蛋白CD34和Ang-1的表达均增加(P<0. 05,P<0. 05)。与模型组相比,莫诺苷大剂量组皮层血管新生相关蛋白CD34和Ang-1的表达显著增加(P<0. 05,P<0. 01),其中莫诺苷中剂量组也能促进Ang-1的表达(P<0. 05)。结论莫诺苷给药时间窗扩大至局灶性脑缺血再灌注后24 h,其大剂量组可以降低神经功能评分,减小脑梗死体积,对CD34及Ang-1血管新生相关蛋白有调节作用。

Objective To investigate the effects of morroniside on angiogenesis-related factors and neurological function in rats24 h after focal cerebral ischemia-reperfusion.Methods Healthy adult male SD rats were induced to develop occlusion of the middle cerebral artery by suture embolus.Then,the rats were randomly divided into a sham group,a model group,a morroniside-low group,a morroniside-intermediate group,and a morroniside low,moderate,and high-dose groups(30,90,and270 mg/kg,respectively).Morroniside was administered24 h after surgery,once a day for 7 days.By determining the scores of neurological behavior,the cerebral infarction volume ratios,and the expression of cortical angiogenesis-related gene proteins,were investigated to explore the effects of morroniside on angiogenesis-related proteins and neurological function in rats at24 h after focal cerebral ischemia-reperfusion.Results Compared with those in the sham group,the scores of neurological behavior and the cerebral infarction volume ratios in the model group were significantly increased(P<0.001).After 7 days of morroniside administration,compared with that in the model group,the morroniside-high dose group exhibited a dramatic improvement of the neurological behavior scores(P<0.01)and a significant reduction in the volume of cerebral infarction(P<0.05).Moreover,the expression of cortical angiogenesis-related proteins CD34 and Ang-1 were significantly increased(P<0.05,P<0.05)compared with those in the sham group.Compared with the model group,the expression of cortical angiogenesis-related proteins CD34 and Ang-1 in the group with high-dose morroniside was significantly increased(P<0.05,P<0.01),and moderate-dose morroniside also promoted the expression of Ang-1(P<0.05).Conclusions The time window for morroniside administration extends to24 h after focal cerebral ischemia-reperfusion.The morroniside administration at a high dose can reduce the neurological behavior scores and the cerebral infarction volume ratio,while increasing the expression of the angiogenesis-related proteins CD34 and Ang-1.