抑制LITAF基因对人脑胶质母细胞瘤U251细胞增殖、凋亡和放疗敏感度的影响

Effect of Inhibiting LITAF Expression on Proliferation, Apoptosis and Radiosensitivity of Human Glioblastoma U251 Cell

目的分析人脑胶质母细胞瘤组织中LITAF基因mRNA表达情况,并通过抑制人脑胶质母细胞瘤U251细胞中LITAF基因表达,观察其对U251细胞增殖、凋亡和放疗敏感度的影响。方法分析TCGA数据库中人脑胶质母细胞瘤组织中LITAF基因mRNA表达;通过RNAi抑制U251细胞中LITAF基因表达,采用胸腺嘧啶核苷类似物(EDU)检测U251细胞增殖变化,流式细胞仪分析U251细胞凋亡的变化,克隆形成实验和流式细胞分析U251细胞放疗敏感度的变化。结果 TCGA数据库分析结果显示人脑胶质母细胞瘤组织LITAF表达显著高于正常脑组织;抑制LITAF表达后,U251细胞的增殖和凋亡未见明显变化,但在电离辐射后,LITAF抑制组U251细胞凋亡显著低于对照组,克隆形成显著高于对照组,对放疗敏感度减弱。结论 LITAF基因高表达于人脑胶质母细胞瘤组织,抑制人脑胶质母细胞瘤U251细胞中LITAF基因表达不影响细胞的增殖和凋亡,但显著降低细胞的放疗敏感度。

Objective To investigate the expression of LITAF in human glioblastoma tissues and the effect of LITAF silence on the proliferation,apoptosis and radiosensitivity of glioblastoma U251 cells.Methods The expression of LITAF in glioblastoma was analyzed in The Cancer Genome Atlas(TCGA)database.The proliferation and apoptosis of U251 cells were detected with EDU kit and flow cytometry after the expression of LITAF was silenced.Clonogenic assay and flow cytometry were performed to assess the radiosensitivity of U251 cells with LITAF RNAi.Results The expression of LITAF was increased significantly in human glioblastoma tissues,compared with normal brain tissues.The proliferation and apoptosis of U251 cells weren't interfered in U251 cells after LITAF knockdown by RNAi.After irradiation,the apoptosis was decreased remarkably and the clone formation was increased markedly in U251 cells with LITAF RNAi,compared with the control cells.Conclusion LITAF is highly expressed in human glioblastoma tissues.Knockdown of LITAF expression has no effect on the proliferation or apoptosis but decreases the radiosensitivity of U251 cells.